165,335 results

1. pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa

   Plasmid

   #221356

   Purpose

   Mammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa; to be used with Anti-Strep [IPI-C23.21] heavy chain (Plasmid 221355) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pU6-Sp-pegRNA-HEK3_CTT_ins

   Plasmid

   #132778

   Purpose

   S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   HEK3_CTT_ins pegRNA
   

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSNA

   Plasmid

   #135192

   Purpose

   Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   I-SceI lamba Red
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Promoter

   araBAD

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTXB1-CfaC-Gyra

   Plasmid

   #122950

   Purpose

   Bacterial expression of C-intein

   Depositor

   Thomas Muir

   Article

   Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

   Insert

   CfaC
   

   Expression

   Bacterial

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGB 35S:Renilla:Tnos (GB0109)

   Plasmid

   #68215

   Purpose

   Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Renilla
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBa.TfR.GFP

   Plasmid

   #45060

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Burack et al Neuron. 2000 May;26(2):465-72.

   Insert

   TfR (TFRC Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Chicken Beta Actin

   Available Since

   Oct. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1 hMOG-alpha1-EGFP

   Plasmid

   #126463

   Purpose

   Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

   Depositor

   Markus Reindl

   Article

   Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

   Insert

   Myelin oligodendrocyte glycoprotein (MOG Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Salsa6f

   Plasmid

   #140188

   Purpose

   Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

   Depositor

   Michael Cahalan

   Article

   Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

   Insert

   Salsa6f
   

   Tags

   tdTomato-v5-GCaMP6f

   Expression

   Mammalian

   Promoter

   CMV Immediate Early

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-∆Hapln1

   Plasmid

   #200778

   Purpose

   Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
   

   Use

   AAV

   Tags

   cfGFP

   Mutation

   deleted lectican binding domain (aminoacids 40-15…

   Promoter

   Synapsin

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUW-tetO-Batf3

    Plasmid

    #139837

    Purpose

    doxycycline-inducible expression of mouse Batf3 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Batf3 (Batf3 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM1 Raptor GFP

    Plasmid

    #112744

    Purpose

    express Raptor-GFP

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Raptor-GFP (RPTOR Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHyg-AID*-6HA

    Plasmid

    #99520

    Purpose

    C-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    6xHA

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE2-SpRY-P2A-GFP

    Plasmid

    #159981

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpRY-P2A-GFP
    

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-F in pAR-9

    Plasmid

    #166921

    Purpose

    AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F

    Depositor

    Casey Maguire

    Article

    Hanlon et al Mol Ther Methods Clin Dev. 2019 Oct 23;1

    Insert

    REP/CAP
    

    Use

    AAV

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

    Plasmid

    #176851

    Purpose

    lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    hYAP1-5SA (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    S61A, S109A, S127A, S164A, S381A (phosphorylation…

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLX1.8NFATGFPpd2HS4

    Plasmid

    #169096

    Purpose

    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

    Depositor

    Geoffrey Owens

    Inserts

    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    

    Use

    Lentiviral

    Promoter

    NFAT enhancer + synthetic TATA box

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pM-CBE

    Plasmid

    #196293

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding CBE in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-DIO-ChR2-TdTomato

    Plasmid

    #166600

    Purpose

    Encodes Cre-dependent ChR2-TdTomato under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    ChR2-TdTomato
    

    Use

    AAV

    Mutation

    H134R

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-TetON-Sox2

    Plasmid

    #110280

    Purpose

    Doxycycline-inducible overexpression of Sox2

    Depositor

    Trudy Oliver

    Article

    Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

    Insert

    SRY (sex determining region Y)-box 2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    No fusion genes

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    TRE

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWM_12x601_15bpLinker

    Plasmid

    #157786

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_15bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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