We narrowed to 27,276 results for: ANT

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pEG302 5aa SunTag VP64 nog

Plasmid

#115480

Purpose

CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 17, 2019

Availability

Academic Institutions and Nonprofits only

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PKAcs-GGSD5X-GFP

Plasmid

#108584

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GW-NL-myc

Plasmid

#113447

Purpose

NanoLuc Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

NanoLuc-cmyc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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LT3REVIN

Plasmid

#111175

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Promoter

T3G

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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p16 GPS1

Plasmid

#101083

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1

Tags

cMyc

Expression

Plant

Promoter

p16 (AT3G60245)

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AR_WT

Plasmid

#82094

Purpose

Gateway Donor vector containing AR , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AR (AR Human)

Use

Gateway Cloning

Mutation

p.81_82insQQ; deletion of AA397-482

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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BAS292 C terminal SF-GFP kanR

Plasmid

#74087

Purpose

S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

SF-GFP recoded for S.pombe

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pETpRha-alpha-SkTm

Plasmid

#72929

Purpose

Allows expression of rabbit alpha-skeletal tropomyosin from a rhamnose inducible promoter

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Insert

alpha skeletal Tropomyosin

Expression

Bacterial

Promoter

rhaB

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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MEK1-pcw107-V5

Plasmid

#64650

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pGC629

Plasmid

#71364

Purpose

proximal somatic gonad expression of DAF-16 in C.elegans

Depositor

Jane Hubbard

Article

Qin et al Nat Commun. 2015 May 11;6:7107. doi: 10.

Insert

daf-16 (daf-16 Nematode)

Tags

GFP(65C)

Expression

Worm

Promoter

fos1a promoter

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CGG-R12-KIRV

Plasmid

#63668

Purpose

CGG-R12-KIRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CGG-R12-KIRV RNAP

Mutation

Q744K, L747V, N748H, L749I, R756E, L757M, H772R, …

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCMB-TMEr

Plasmid

#61208

Purpose

Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry-BCP

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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3I91

Plasmid

#62514

Purpose

Bacterial expression plasmid for use in structural determination.

Depositor

Cheryl Arrowsmith

Article

Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

Insert

CBX8 (CBX8 Human)

Tags

6xHis and TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pSYN-PC7-006

Plasmid

#42502

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

PC7-SOL CDS (PCSK7 Human)

Expression

Mammalian

Promoter

cmv

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-EGFAB(H306Y)

Plasmid

#25771

Depositor

Johann Deisenhofer

Article

McNutt et al J Biol Chem. 2009 Apr 17. 284(16):10561-

Insert

LDL receptor EGFAB (LDLR Human)

Tags

GST

Expression

Bacterial

Mutation

His 306 to Tyr

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-GFP/CALR del52-FLAG

Plasmid

#204523

Purpose

Retroviral expression of human CALR del52-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR del52-FLAG (CALR Human)

Use

Retroviral

Mutation

del52

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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2XSmBiT-SARS-CoV-2 S1

Plasmid

#173430

Purpose

protein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 Fc

Depositor

David Nemazee

Article

Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

Insert

2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)

Use

Luciferase

Tags

human IgG1 Fc

Expression

Mammalian

Mutation

furin cleavage site RRAR to GSAS

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

Plasmid

#160624

Purpose

TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:MS2:VPR:Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD mApple

Plasmid

#91772

Purpose

Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

monomeric Apple

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

R97K, E152S, A180S, V207T compared to mApple (FP …

Promoter

araBAD

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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RP78 pUAST Orco mRNA

Plasmid

#53431

Purpose

Expresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS control

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

Orco (Orco )

Expression

Insect

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only