We narrowed to 12,476 results for: pcdna
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FLAG-MED31
Plasmid#15427DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
CFP-GIV-CT WT
Plasmid#69532PurposeMammalian expression of The CFP-tagged GIV-CT biosensorsDepositorInsertGIV (CCDC88A Human)
TagsCFPExpressionMammalianMutationC terminal amino acids S1660-S1870 of human GIVPromoterCMVAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-FLAG
Plasmid#105783PurposeExpression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-FLAGExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE4565
Plasmid#88903PurposeExpresses inactive/dead, humanized MbCpf1 nucleaseDepositorInserthMbCpf1(D986A)
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMVAvailable SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Opto-GPR17
Plasmid#106005PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR17; Opto-GPR17; A7)DepositorInsertOpto-GPR17 (GPR17 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
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FKBP-DCRD-YFP
Plasmid#87450Purposerapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channelsDepositorInsertDCRD (distal C-terminal regulatory domain) (CACNA1F Human)
Tags6xHis-T7, FKBP, and YFPExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-YFP
Plasmid#153544Purposeperform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publicationDepositorInserthuman TCR ζ-Chain (CD247 Human)
TagsVenus YFPExpressionMammalianMutationwith mCherry in LIC-Z replaced by YFPPromoterCMVAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTE4567
Plasmid#107557PurposeExpresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.DepositorInserthMbCpf1(dead2)
Tags3xHA and NLSExpressionMammalianMutationE1080APromoterCMVAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS
Plasmid#59388PurposeDestination vector with RBPMS for stable cell line generationDepositorAvailable SinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
FR_HNF1B_A263insGG
Plasmid#31103DepositorAvailable SinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR19
Plasmid#106007PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR19; Opto-GPR19; A9)DepositorInsertOpto-GPR19 (GPR19 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL681 CHYRON4 integration construct
Plasmid#126449PurposeTo integrate the CHYRON4 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON4 hgRNA
pCMV-puro
ExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C211
Plasmid#174360PurposeFor expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfectionDepositorInsertPABPC1 (PABPC1 Human)
TagsFLAGExpressionMammalianMutationI110L, D111E, D117E, A121G, G139A, Y140F, T147S, …Available SinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-human-BNIP3 delta Exon2
Plasmid#100782PurposeMammalian expression of BNIP3 delta Exon2 splice variantDepositorInserthuman-BNIP3 delta Exon2 (BNIP3 Human)
TagsHAExpressionMammalianMutationExon2 deletionPromoterCMVAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
FR_HNF1B_P328L329del
Plasmid#31102DepositorInsertHNF1B (HNF1B Human)
UseFlp/frtTagsmycExpressionMammalianMutationmutation P328L329delCCTCTAvailable SinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRD397
Plasmid#163104PurposeExpression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_mTurquoise2_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
CFP-PCRD-FRB
Plasmid#87449Purposerapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channelsDepositorInsertPCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
Tags6xHis-T7, CFP, and FRBExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRD439
Plasmid#168465PurposeFor the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mTurquoise2-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
1029-AIDx-CP
Plasmid#135341PurposeExpression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.DepositorInsertCP 1029 nSpyCas9-hAIDx
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AIDx-ID-1058
Plasmid#135347PurposeExpression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminusDepositorInsertnSpyCas9-hAIDx-ID-1058-UGI
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR173
Plasmid#106058PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR173; Opto-GPR173; E12)DepositorInsertOpto-GPR173 (GPR173 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR31
Plasmid#106014PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR31; Opto-GPR31; B4)DepositorInsertOpto-GPR31 (GPR31 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR61
Plasmid#106027PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR61; Opto-GPR61; C5)DepositorInsertOpto-GPR61 (GPR61 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-EOMESwt-IDR
Plasmid#145231PurposeExpresses fusion of GAL4 DNA-binding domain and EOMESwt IDRDepositorInsertEOMESwt-IDR (EOMES Human)
ExpressionMammalianAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
USPQ-HTT-TALEN1
Plasmid#92243PurposeTALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2DepositorInsertTALEN
UseTALENPromoterCMVAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
USPQ-HTT-TALEN2
Plasmid#92244PurposeTALEN targeting upstream of HTT gene (ELD), forms obligate heterodimer with USPQ-HTT-TALEN1DepositorInsertTALEN
UseTALENPromoterCMVAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-BHLHE41wt-IDR
Plasmid#145229PurposeExpresses fusion of GAL4 DNA-binding domain and BHLHE41wt IDRDepositorInsertBHLHE41wt-IDR (BHLHE41 Human)
ExpressionMammalianAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only
