165,343 results

1. pCBEclos-cbei2385-g2-opt

   Plasmid

   #118215

   Purpose

   CRISPR-mediated base editing in Clostridium

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol Bioeng. 2019 Feb 10. doi: 10.

   Insert

   Apobec1, Casp nickase, UG1-sgRNA
   

   Expression

   Bacterial

   Promoter

   Ptm

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR

   Plasmid

   #195853

   Purpose

   Expresses inhibitory opsin gtACR2 and GFP in turning associated neurons

   Depositor

   Andrew Leifer

   Article

   Kumar et al PLoS Biol. 2023 Sep 21;21(9):e3002280. d

   Insert

   Guillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
   

   Expression

   Worm

   Promoter

   lim-4

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ubi:Zebrabow

   Plasmid

   #118219

   Purpose

   For ubiquitous expression of Brainbow in zebrafish

   Depositor

   Alex Schier, Albert Pan

   Article

   Pan et al Development. 2013 Jul;140(13):2835-46. d

   Insert

   Brainbow 1.0 L
   

   Use

   Zebrafish expression

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. XPO6-EGFP

   Plasmid

   #210371

   Purpose

   Expresses XPO6 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   XPO6 (XPO6 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-AN-HA_WT POLH

   Plasmid

   #201671

   Purpose

   Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   Polymerase eta (POLH Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR-attL5-CRY2-mCh-attL2

   Plasmid

   #160438

   Purpose

   Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Cryptochrome 2 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.2 V5-DEST 3XFlag-SRSF3

   Plasmid

   #46736

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   SRSF3 (SRSF3 Human)
   

   Tags

   3x Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLM1110_LEU2

   Plasmid

   #168460

   Purpose

   Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

   Depositor

   Jef Boeke

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Insert

   mRFP1
   

   Use

   Synthetic Biology; Yeast assembly vector for big-…

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Halo-Sec61-C-18

   Plasmid

   #123285

   Purpose

   Localization: Endoplasmic Reticulum

   Depositor

   Kevin McGowan

   Insert

   Sec61 (SEC61B Human)
   

   Tags

   HaloTag7

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHyg-AID*-6HA

    Plasmid

    #99520

    Purpose

    C-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    6xHA

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3 3 kb prom. CD274

    Plasmid

    #107004

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCZGY2254

    Plasmid

    #101247

    Purpose

    GFP subunits 1-10, (only shows active fluorescence when binds to GFP11)

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    gfp1-10
    

    Use

    Gateway

    Tags

    GFP1-10

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKSI-1

    Plasmid

    #51725

    Purpose

    cloning backbone

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-FLAG-Luciferase

    Plasmid

    #58792

    Purpose

    Express FLAG-tagged Luciferase in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    firefly Luciferase
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

15. qTAG-C-V5-Puro

    Plasmid

    #207730

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. GFP-KASH2

    Plasmid

    #187017

    Purpose

    Inducible disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    KASH2
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-ERKKTRClover

    Plasmid

    #59138

    Purpose

    ORF encoding ERK KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
    

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3-EF1aH N90-beta-catenin

    Plasmid

    #86499

    Purpose

    Mammalian expression of Myc tagged N90 beta-catenin

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90; constitutively acti…

    Promoter

    EF1a

    Available Since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIS1

    Plasmid

    #12179

    Depositor

    David Bartel

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pD649-HAsp-HLA-E-COMP5AP-AviTag-9xHis

    Plasmid

    #157396

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    HLA-E (HLA-E Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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