We narrowed to 13,692 results for: Egf

1. pmyc-GFP-TNRC6A-NLS/NES-mut

   Plasmid

   #42002

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   R930A, R931A, R932A, R934A, F951A, F955A, I958A, …

   Promoter

   CMV

   Available since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA-T-intron(Neo)-G(FLLFLNL)

   Plasmid

   #40021

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   T (ATG)
   GFP
   beta-globin intron
   Neo
   

   Use

   Tags

   Expression

   Mutation

   deleted nucleotides 1-274 and insertion of FRT, …

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCellFree_G03 FYN

   Plasmid

   #67106

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   FYN (FYN Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pTK157_mCherry-4.1G-CTD

   Plasmid

   #46359

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   4.1G C-terminal domain (EPB41L2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C-terminal domain from 886-1005 amino acids

   Promoter

   CMV

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. FHp5pUP95C3,5SGW (E5)

   Plasmid

   #74035

   Purpose

   lentiviral expression of Psd95 shRNA and Psd95-C3,5S-EGFP fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   PCR: C3,5S (tct aga cca cca tgg act Ctc tAt CGa t…

   Promoter

   H1

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pLib6.4-Aalb_726

   Plasmid

   #176661

   Purpose

   Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLib6.4-Aaeg_763

   Plasmid

   #176658

   Purpose

   Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFasBac+GFP-CAMSAP2 CC+CKK

   Plasmid

   #59039

   Purpose

   for baculovirus expression of CAMSAP2 CC+CKK in Sf9 cells

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP2 CC+CKK domains (CAMSAP2 Human)
   

   Use

   Tags

   His and eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46593

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-16 Decoy (Mir16-1 Human, Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCellFree_G03 CREBZF

    Plasmid

    #67070

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CREBZF (CREBZF Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pGFP-Ub-VPS13D_dC

    Plasmid

    #176466

    Purpose

    express VPS13D lacking C-terminal 407 aa in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    C-terminal 407 aa is deleted.

    Promoter

    CMV

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-GFP-Patronin CC+CKK

    Plasmid

    #59048

    Purpose

    for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pENTR-GFP-Patronin CC 534–1457

    Plasmid

    #59050

    Purpose

    for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pInducer21 Flag-Sesn2

    Plasmid

    #61829

    Purpose

    Dox inducible lentivirial expression of Flag-Sesn2 (mouse)

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn2 (Sesn2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pNMHCII-C1C2-GFP

    Plasmid

    #46044

    Depositor

    Robert Adelstein

    Article

    Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

    Insert

    nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCellFree_G03 CSNK2A1

    Plasmid

    #67085

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. UAS-Xbp1-GFP

    Plasmid

    #80627

    Purpose

    expression of Xbp1-GFP (RA isoform) in Drosophila

    Depositor

    Pedro Domingos, Herman Steller

    Article

    Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

    Insert

    Xbp1 (Xbp1 Fly)
    

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    RA isoform

    Promoter

    UAS

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

18. mGFAP R236H

    Plasmid

    #169475

    Purpose

    Single p.Arg236His(R236H) mutation was individually inserted by site-directed mutagenesis into the wild-type mGFAP

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    HaloTAG mGFAP (R236H) (Gfap Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed R 236 to H

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ptagBFP-TetR-NLS-VP16

    Plasmid

    #103835

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-TetR-NLS-VP16 (tetR )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV2

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. cmamxA1-GFP

    Plasmid

    #107195

    Purpose

    Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    cmannexin A1
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMTG03-AmpFRT

    Plasmid

    #128272

    Purpose

    TIP-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP---CMV-TetOx2-GFP

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Inserts

    TetR-LOV-TIP
    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMTG05-Amp-FRT

    Plasmid

    #128274

    Purpose

    TIP-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-GFP

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Inserts

    TetR-LOV-TIP
    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMTG04-AmpFRT

    Plasmid

    #128273

    Purpose

    Deg-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG---CMV-TetOx2-GFP

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Inserts

    TetR-LOV-RRRG
    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Py2087ACR1

    Plasmid

    #153032

    Purpose

    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

    Insert

    Py2087ACR1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+ enhancer)

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCellFree_G03 CTNNB1

    Plasmid

    #67119

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pGL3-U6-sgRNA_EMX1-mcherry

    Plasmid

    #159784

    Purpose

    S. pyogenes sgRNA collocated with pegRNA targeting human EMX1 gene

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of sgRNA targeting EMX1gene (EMX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJOE6089.4

    Plasmid

    #135081

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-eGFP-Strep-tag II
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBEST-HipO

    Plasmid

    #114599

    Purpose

    Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    HipO
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. OVA-miSFIT-19-G

    Plasmid

    #124676

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCALNL_chr13_102010574-102010650

    Plasmid

    #81213

    Purpose

    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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