161,273 results

1. pJet-CMV-IARS1[W435C]-GFP

   Plasmid

   #212323

   Purpose

   EGFP-tagged IARS1 Trp435Cys

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   IARS (IARS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Trp435cys

   Promoter

   CMV

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJET-CMV-GFP-P2A-K20-P2A-mCherry

   Plasmid

   #185618

   Purpose

   Ribosomal stalling reporter: positive control

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   GFP-K20(AAA)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJET-CMV-GFP-P2A-K0-P2A-mCherry

   Plasmid

   #185619

   Purpose

   Ribosomal stalling reporter: empty

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   GFP-empty-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJET-CMV-GFP-P2A-I15-P2A-mCherry

   Plasmid

   #185620

   Purpose

   Ribosomal stalling reporter: 15x isoleucine

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   GFP-I15-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-mGFAP-Lck-PinkFlamindo

   Plasmid

   #228394

   Purpose

   AAV vector to express the red cAMP indicator in astrocytes

   Depositor

   Tetsuya Kitaguchi

   Article

   Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

   Insert

   Pink Flamindo
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   mGFAP

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRXIB-mRuby3-STX17TM

   Plasmid

   #221744

   Purpose

   Stable expression of mRuby3-STX17TM in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   STX17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-228

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDC180

   Plasmid

   #218673

   Purpose

   Expresses rat ⍺SNAP in bacteria

   Depositor

   Axel Brunger

   Article

   Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

   Insert

   rat ⍺SNAP (Napa Rat)
   

   Use

   Tags

   6 his

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.23_Promoter_4

   Plasmid

   #210630

   Purpose

   Luciferase experiment for TAL1 promoter 4

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   promoter 4 (TAL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal Promoter

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.23_exon 4

   Plasmid

   #210631

   Purpose

   Luciferase experiment for TAL1 exon 4

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   TAL1 exon 4 (TAL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal Promoter

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF1606

    Plasmid

    #143829

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BAZ2A (BAZ2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF1717

    Plasmid

    #142834

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    LDB1 (LDB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTR-340 pUC19-tNGFR-P2A-IL2RG N

    Plasmid

    #186067

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL2RG (IL2RG Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-mCherry-PA-Rac1

    Plasmid

    #169042

    Purpose

    Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    PA-Rac-1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Gateway entry cloning vector

    Tags

    6X His and Fused with mCherry

    Expression

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

    Plasmid

    #164013

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R464E

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

    Plasmid

    #163991

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a human Tankyrase2 full length Y920E

    Plasmid

    #132639

    Purpose

    expresses human Tankyrase2 full length Y920E in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

    Insert

    Tankyrase2 Y920E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. ninaE-norpA

    Plasmid

    #26854

    Depositor

    Craig Montell

    Article

    Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

    Insert

    no receptor potential A (norpA Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    V963A

    Promoter

    Available since

    April 5, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pDIRECT_23C

    Plasmid

    #91140

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRetroQ-Cre-ERT2

    Plasmid

    #59701

    Purpose

    to make Cre-ERT2 expressing stable cell line by retrovirus

    Depositor

    Richard Youle

    Insert

    Cre-ERT2
    

    Use

    Retroviral

    Tags

    Estrogen receptor (ERT)

    Expression

    Mammalian

    Mutation

    Promoter

    cmvIE

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

    Plasmid

    #224435

    Purpose

    AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mScarlet-KASH
    U6-gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hCathepsin L

    Plasmid

    #11250

    Purpose

    Mammalian expression of human cathepsin L

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2005 Dec 8. ():.

    Has Service

    Cloning Grade DNA

    Insert

    cathepsin L (CTSL Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pTwist-SARS-CoV-2 Spike-deltaC18 LB.1

    Plasmid

    #223277

    Purpose

    Encodes SARS-CoV-2 spike LB.1 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike LB.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FASTHDR-Cterm-mClover3-Puromycin

    Plasmid

    #167205

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA4-Chk1-Flag

    Plasmid

    #22894

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Chk1 (CHEK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHyg-AID*-GFP

    Plasmid

    #99521

    Purpose

    C-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CMV-TO-scHCVP

    Plasmid

    #116088

    Purpose

    Expresses an engineered HCV protease

    Depositor

    Michael Elowitz

    Article

    Gao et al Science. 2018 Sep 21;361(6408):1252-1258

    Insert

    scHCVP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. lentiCRISPR v2 sgKEAP1-3

    Plasmid

    #186837

    Purpose

    knock out KEAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EGFP-Rab7A Q67L

    Plasmid

    #28049

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    2xHA and EGFP

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    Available since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEMT-3P2A

    Plasmid

    #111772

    Purpose

    Quadcistronic construct: Co-express four genes by 3 P2A peptides

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Type

    Empty backbone

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCW57.1 Lysosomal-METRIQ

    Plasmid

    #135401

    Purpose

    Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

    Depositor

    Eisuke Itakura

    Article

    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

    Insert

    DNAse II alpha (DNASE2 Human)
    

    Use

    Lentiviral

    Tags

    T2A, mCherry, and sfGFP

    Expression

    Mammalian

    Mutation

    Single-nucleotide polymorphism C432T

    Promoter

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge