163,445 results

1. pcDNA3-myc3-CUL4B

   Plasmid

   #19922

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   Cullin 4B (CUL4B Human)
   

   Use

   Tags

   3xmyc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-neo-mOVA

   Plasmid

   #25099

   Depositor

   Maria Castro

   Article

   Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

   Insert

   membrane bound ovalbumin
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPM-miR30-IFIH1-ts2

   Plasmid

   #174260

   Purpose

   IFIH1 knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   IFIH1 shRNA (IFIH1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-minP:luc2

   Plasmid

   #138354

   Purpose

   Gateway compatible middle entry clone contains Firefly Luciferase2 insert driven by minimal promoter

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-N BNLF2a

   Plasmid

   #37941

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   BNLF2a (BNLF2a H. Herpesvirus 4 (EBV))
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   PKG

   Available since

   Aug. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. DJ-1 pET15b

   Plasmid

   #60678

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

   Insert

   DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pWXC078

   Plasmid

   #222961

   Purpose

   C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

   Depositor

   Oliver Hobert

   Article

   Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

   Insert

   mScarlet-I3
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-R-Rac1

   Plasmid

   #180576

   Purpose

   ddRFP sensor of Rac1

   Depositor

   Won Do Heo

   Article

   Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

   Insert

   ddRFP sensor of Rac1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

   Plasmid

   #104495

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, and AAV1

   Insert

   jGCaMP7s
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBMN mEGFP-NDP52

    Plasmid

    #188785

    Purpose

    For mammalian expression of NDP52 tagged with mEGFP

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLM-CMV-R-Cre

    Plasmid

    #27546

    Purpose

    Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

    Insert

    mCherry_P2A_Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLminP_Luc2P_RE43

    Plasmid

    #90381

    Purpose

    RUNX - Runx1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    GATA3

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGC-Lb12-P1Bb

    Plasmid

    #231387

    Purpose

    For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

    Depositor

    Qi-Jun Chen

    Article

    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

    Insert

    U6-29
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. NLS-Laconic/pCMV-myc-nuc

    Plasmid

    #46307

    Purpose

    a nuclear-targeted, genetically-encoded sensor for lactate

    Depositor

    Luis Felipe Barros

    Article

    Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

    Insert

    NLS-Laconic
    

    Use

    Lactate biosensor

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTD103luxI_sfGFP

    Plasmid

    #48885

    Purpose

    Positive feedback plasmid from oscillator

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    LuxI, sfGFP, and LuxR
    

    Use

    Synthetic Biology

    Tags

    ssrA tag (AANDENYALAA)

    Expression

    Bacterial

    Mutation

    Promoter

    pLux

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBJ FLAG-hPar1

    Plasmid

    #53226

    Purpose

    mammalian expression of human Par1 with N-terminal FLAG tag

    Depositor

    Shaun Coughlin

    Article

    Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

    Insert

    Par1 (F2R Human)
    

    Use

    Tags

    FLAG and bovine prolactin signal sequence (MDSKGS…

    Expression

    Mammalian

    Mutation

    Deletion of aa1-39 (native signal peptide)

    Promoter

    SV40

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-MXT GIRK2

    Plasmid

    #20675

    Depositor

    Henry Lester

    Article

    Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

    Insert

    Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
    

    Use

    Xenopus expression

    Tags

    3' UTR Xenopus B- globin and 5' UTR Xen…

    Expression

    Mutation

    A369V

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

    Plasmid

    #67986

    Purpose

    Cas9 activity reporter with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19/PLIN2 GFP-P2A-BFP

    Plasmid

    #202195

    Purpose

    knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP-P2A-BFP knock-in

    Expression

    Mutation

    Promoter

    lac

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. plenti-UbcP-FLAG-CRBN-pGK-HYG

    Plasmid

    #107374

    Purpose

    Lentiviral expression of FLAG-CRBN

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    UbcP

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAGGS-PHFF-sh-hBVRA

    Plasmid

    #100285

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    

    Use

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEGFP-Rubicon

    Plasmid

    #21636

    Depositor

    Tamotsu Yoshimori

    Article

    Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

    Insert

    Rubicon (1700021K19Rik Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. R4pGWB601_UBQ10p-Turbo-NES-YFP

    Plasmid

    #127366

    Purpose

    Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GLASTp-DsRed2

    Plasmid

    #17706

    Depositor

    Nicholas Gaiano

    Article

    Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

    Insert

    GLASTp (SLC1A3 Human)
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3 Flag-humanTRAF6 (1093)

    Plasmid

    #66929

    Purpose

    Mammalian Expression of TRAF6

    Depositor

    Michael Karin

    Insert

    TRAF6 (TRAF6 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TPC1-G-GECO1.2

    Plasmid

    #207143

    Purpose

    Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPCN1 (TPCN1 Human)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LentiGuide-10X-CS1-3'-puro

    Plasmid

    #212011

    Purpose

    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)

    Depositor

    Danwei Huangfu

    Insert

    gRNA scaffold
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TACR3-Tango

    Plasmid

    #66516

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    TACR3 (TACR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

29. gCH27 (crCD55-4)

    Plasmid

    #217343

    Purpose

    crRNA targeting CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crCD55-4 (CD55 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-RalGDS

    Plasmid

    #118315

    Purpose

    Expression of human RalGDS

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    RalGDS (RALGDS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    fragment of human RalGDS 203aa

    Promoter

    CMV

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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