172,546 results
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Plasmid#54605PurposeLocalization: C1 Cloning Vector, Excitation: 433, Emission: 475DepositorTypeEmpty backboneTagsmCerulean3ExpressionMammalianAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only
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pB_35S/mEGFP
Plasmid#135320PurposemEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)DepositorInsertmEGFP
ExpressionPlantPromoterCauliflower mosaic virus 35SAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
YY206: pAAV_hSyn::GEMINI(B)-p2A-GEMINI(A)_UbC::HaloTag-GEMINI(A)
Plasmid#228886PurposeAAV plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a hSyn promoter, and HaloTag-GEMINI(A) under the control of a UbC promoter.DepositorInsertGEMINI(A); GEMINI(B); HaloTag-GEMINI(A)
UseAAVTagsHaloTagExpressionMammalianPromoterhSyn; UbcAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K29
Plasmid#22903DepositorInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationK29 only, all other lysines mutated to argininesAvailable SinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
GPI-RpHLuorin2
Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorInsertRatiometric pHLuorin2 fused to GPI anchor
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL11017 (pICH47732::NOSp-BAR-NOST)
Plasmid#51145PurposeConfers Basta resistance in plantaDepositorInsertNOSp-BAR-NOST
Available SinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTomatoSlicer
Plasmid#221085PurposeDual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-alpha
Plasmid#88846PurposeCRISPR KO of Trp53DepositorAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
LV-Cre pLKO.1
Plasmid#25997PurposeLentiviral expression of nls-Cre from the CMV promoterDepositorInsertCre recombinase
UseCre/Lox and LentiviralExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
4xmts-mScarlet-I
Plasmid#98818PurposeIn vivo visualization of mitochondria (can be used for colocalization studies)DepositorInsertmts
TagsmScarlet-IExpressionMammalianPromoterCMVAvailable SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ153
Plasmid#69304PurposeGateway entry vector with pco-dCas9-3X(SRDX)DepositorInsertpco-dCas9-3X(SRDX) (Plant codon-optimized)
UseCRISPRTags2XFLAGMutationSRDX (X3) repressor fusion; NLS fusionAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT
Plasmid#87259PurposeYFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133DepositorInsertsYFP-mAID-3HA
HXGPRT
UseToxoplasma expressionTags3HA and mAIDMutationCodon optimized for Toxoplasma gondii expressionPromoterTgDHFR-TS 463 bp and TgTUB1 378 bpAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1365 LV EF1a-CD28 IRES-EGFP
Plasmid#201921Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD28DepositorAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLGB29
Plasmid#126619PurposeSuicide vector for allelic replacement in Bacteroides, inulin selection and aTC-inducible ss-Bfe1 counterselectionDepositorTypeEmpty backboneUseAllellic exchange in bacteriaAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a_6H-MMLV_RT_D524N-6H
Plasmid#166945PurposeBacterial expression of M-MLV reverse transcriptaseDepositorInsertgag-pol
Tags6X HisExpressionBacterialMutationD524N, H8YPromoterT7Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-beta
Plasmid#88847PurposeCRISPR KO of Trp53DepositorAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS578_DD-dSpyCas9-mCherry-APEX2
Plasmid#108570PurposeExpression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factorsDepositorInsertdSpyCas9
UseCRISPR and LentiviralTagsAPEX2, Destabilization domains, Flag, and mCherry…ExpressionMammalianPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-NLS-HA
Plasmid#141321PurposePlasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)DepositorInsertRfx-Cas13d (NLS-RfxCas13d-NLS)
UseCRISPRTagsHA and NLSAvailable SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-puro_Gateway-3xFLAG
Plasmid#172981PurposeGateway Destination vector for doxycycline-inducible expression of ORFDepositorTypeEmpty backboneUseLentiviralTags3xFLAGExpressionMammalianAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-25xUPRE-minP-EGFP
Plasmid#159668PurposeEGFP reporter plasmid containing 25 tandem repeats of the the unfolded protein response element (UPRE)DepositorInsert25xUPRE-minP-EGFP
UseLentiviralExpressionMammalianAvailable SinceMarch 5, 2021AvailabilityAcademic Institutions and Nonprofits only