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Plasmid#159808PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pNM3823
Plasmid#154123Purposemec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vectorDepositorInsertmec-4p::tetR-L-QFAD::act-4 '3
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOD1988-epiDEG
Plasmid#89357PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPdpy-7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXF89
Plasmid#139030PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInserthalotag (no STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOD2046-bwmDEG
Plasmid#89367PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPmyo-3Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK2014
Plasmid#171931PurposeTo express sygl-1 fused to 24 copies of MS2 loops.DepositorInsertPsygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR
UseTagsN-terminal Tag 24X MS2 loopsExpressionWormMutationPromoter-Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD286 (TagRFP^SEC^3xMyc)
Plasmid#70684PurposeTagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.DepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized TagRFP-TExpressionWormMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOD2044-intDEG
Plasmid#89366PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPelt-2Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
Plasmid#159859PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCM1.128
Plasmid#21385DepositorInsertspe-11 promoter (no ATG) in pDONRP4P1R
UseGatewayTagsExpressionMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOD2047-bwmControl
Plasmid#100006PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2046-bwmDEG. Expression is controlled by Pmyo-3 (body wall muscle specific).DepositorInsertsUseTargeting vector for mos1 transposon mediated sin…TagsExpressionMutationPromoterAvailable sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXF276
Plasmid#139048PurposeMosSCI SapTrap assembly: Cassette 3B for 5-cassette systemDepositorInsertlov domain (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJF5
Plasmid#139031PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInsertgfp (no STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSDH52
Plasmid#139049PurposeMosSCI SapTrap assembly: Cassette 3B for 5-cassette systemDepositorInsertepdz (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
Plasmid#158967PurposeUbiquitous expression of mtON in C. elegansDepositorInserteft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
UseTagsGFPExpressionWormMutationPromotereft-3Available sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGH8 - pRAB-3::mCherry::unc-54utr
Plasmid#19359DepositorArticleInsertPrab-3::mCherry
UseTagsmCherryExpressionWormMutationPromoterAvailable sinceOct. 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14_sur5p-dCas9-VP64
Plasmid#177788PurposeOverexpression of dCas9-VP64 in C. elegansDepositorInsertdCas9-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only