1,862 results

1. pMDJ231 - Phsp Cas9

   Plasmid

   #191382

   Purpose

   Heat shock inducible Cas9 expression in C. elegans.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Phsp-16.41 Cas9 gpd-2 tagRFP-t
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Phsp-16.41

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF11-wmiRFP713-T2A-HO1

   Plasmid

   #197246

   Purpose

   Expresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegans

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wmiRFP713-T2A-HO1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA122 - peel-1 negative selection

   Plasmid

   #34873

   Purpose

   Negative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   peel-1 (peel-1 Nematode)
   

   Use

   Worm targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNMSB104

   Plasmid

   #199314

   Purpose

   MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

   Depositor

   Michael Krieg

   Article

   Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

   Insert

   flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mTagBFP2 from pJJR81, C. elegans codon optimized …

   Promoter

   flp-18

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPK605

   Plasmid

   #38148

   Depositor

   Patricia Kuwabara

   Inserts

   unc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR) (unc-119 Nematode)
   pie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   pie-1 promoter and unc-119

   Available since

   Oct. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF11-wmiRFP680-T2A-HO1

   Plasmid

   #197244

   Purpose

   Expresses the protein of wmiRFP680-T2A-HO1 in neurons of C elegan

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wmiRFP680-T2A-HO1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJW1310

   Plasmid

   #61253

   Purpose

   Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   U6 promoter
   

   Use

   CRISPR; Cloning template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

   Plasmid

   #159807

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSF11-wmCardinal

   Plasmid

   #197251

   Purpose

   Expresses the protein of wmCardinal in neurons of C. elegans

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wmCardinal
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSF11-wElectra2

    Plasmid

    #184933

    Purpose

    Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    tag-168

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFJ150 - pDESTttTi5605[R4-R3]

    Plasmid

    #19329

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    pDESTttTi5605[R4-R3]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAD43-daf-16 RNAi

    Plasmid

    #34833

    Depositor

    Cynthia Kenyon

    Article

    Dillin et al Science. 2002 Oct 25;298(5594):830-4.

    Insert

    daf-16 (daf-16 Nematode)
    

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pJW2098

    Plasmid

    #163094

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

    Plasmid

    #159899

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | tagRFP | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSF11-wmTagBFP2

    Plasmid

    #184934

    Purpose

    Codon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmid

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    tag-168

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCFJ601 - Peft-3 Mos1 transposase

    Plasmid

    #34874

    Purpose

    Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    Mos1 transpoase
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGH8 - pRAB-3::mCherry::unc-54utr

    Plasmid

    #19359

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Prab-3::mCherry
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pdestDD04Neo

    Plasmid

    #26386

    Depositor

    Denis Dupuy

    Article

    Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSEZ-1

    Plasmid

    #51868

    Purpose

    Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

    Depositor

    Meng-Qiu Dong

    Article

    Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

    Insert

    psdhb-1::mtLS-cpYFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    sdhb-1

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET_MBP-RGG-GFP-RGG

    Plasmid

    #124949

    Purpose

    Expression of MBP-RGG-GFP-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    EGFP, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge