We narrowed to 303 results for: bmf

1. pCMV-Neo-Bam p53 R248W

   Plasmid

   #16437

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 R248W (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R248W

   Promoter

   Available since

   Aug. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMKO.1 puro p53 shRNA 1

   Plasmid

   #10671

   Depositor

   William Hahn

   Article

   Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

   Insert

   p53 shRNA (TP53 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-Neo-Bam p53 R249S

   Plasmid

   #16438

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 R249S (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R249S

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p11d-aRPA (MSW#712)

   Plasmid

   #208078

   Purpose

   Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

   Depositor

   Marc Wold

   Article

   Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

   Insert

   RPA1, RPA4, RPA3 (RPA1, RPA3, RPA4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3 p53 S15A

   Plasmid

   #69004

   Purpose

   mammalian expression of human p53 S15A mutant.

   Depositor

   David Meek

   Article

   Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S15A

   Promoter

   CMV

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3 p53 S15D

   Plasmid

   #69005

   Purpose

   mammalian expression of human p53 S15D mutant

   Depositor

   David Meek

   Article

   Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S15D

   Promoter

   CMV

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

7. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

   Plasmid

   #86843

   Purpose

   HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

   Depositor

   Thomas Cech

   Article

   Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

   Insert

   FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
   

   Use

   Tags

   FLAG-HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous TERT promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

   Plasmid

   #198863

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human TERT gene

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   TERT sgRNA (TERT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOmniBac zz TEV YBBR POT1

   Plasmid

   #185444

   Purpose

   Expresses human POT1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

   Depositor

   Ahmet Yildiz

   Article

   Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

   Insert

   POT1 (POT1 Human)
   

   Use

   Tags

   YBBR and ZZ

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMKO.1 puro p53 shRNA 2

    Plasmid

    #10672

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 145-pGL3ctrl-3' UTR

    Plasmid

    #28175

    Depositor

    Michael Kastan

    Article

    Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

    Insert

    Luciferase flanked by tp53 UTRs (TP53 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Note that insert length is based on sum of the 2 …

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-p53 R175H

    Plasmid

    #39480

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Insert

    TP53 (TP53 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    P72R and R175H

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

13. HR-TERT-promotor

    Plasmid

    #71398

    Purpose

    Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE2-EF1a-MCS-hPOT1_IRES-blast

    Plasmid

    #166411

    Purpose

    express hPOT1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    ef-1a

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET23 P53DBD

    Plasmid

    #174503

    Purpose

    express P53 DNA binding domain (94-312) in E.coli without tag

    Depositor

    Stewart Loh

    Article

    Butler et al Biochemistry. 2003 Mar 4;42(8):2396-403.

    Insert

    P53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p53-(1-393)-R273H-N268D

    Plasmid

    #35087

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1-393 - R273H-N268D

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

17. p53-(1-393)-R175H-N268D

    Plasmid

    #35086

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1-393 - R175H-N268D

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

18. p53-(1-393)-E285K-N268D

    Plasmid

    #35089

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Contains aa 1-393 with N269D and E285K mutations

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLPC-N-Myc-Flag-BirA-hPOT1

    Plasmid

    #166409

    Purpose

    express BirA-hPOT1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Use

    Tags

    BirA, flag, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast

    Plasmid

    #166412

    Purpose

    express hPOT1 K90E in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 K90E (POT1 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    K90E

    Promoter

    EF-1a

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p53-(1-393)-E285A-N268D

    Plasmid

    #35090

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1-393 - E285A-N268D

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

22. p53-(1-393)-E285V-N268D

    Plasmid

    #35088

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1-393 - E285V-N268D

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pAc5.1-PH-mCherry-human p53 1-50 (HK180)

    Plasmid

    #206458

    Purpose

    p53 1-50 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    p53 1-50 (TP53 Human)
    

    Use

    Tags

    PH mcherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pWZL N-Myc-hPOT1_K90E-IRES_hygro

    Plasmid

    #166407

    Purpose

    Express hPOT1 K90E in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K90E

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV-hTERT-IRES-hygro

    Plasmid

    #85140

    Purpose

    Lentiviral expression of hTERT, used to create immortalized cell lines

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCI neo-hEST2-HA

    Plasmid

    #1782

    Depositor

    Bob Weinberg

    Article

    Meyerson et al Cell 1997 Aug 22;90(4):785-95.

    Insert

    hTERT (TERT Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Clone is catalytically active but is dead in vivo.

    Promoter

    Available since

    July 10, 2005

    Availability

    Academic Institutions and Nonprofits only

27. HIS-HA-CMYC P53

    Plasmid

    #233738

    Purpose

    expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

    Depositor

    Albert Keung

    Article

    Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

    Insert

    HIS-HA-CMYC-p53 (TP53 Human)
    

    Use

    Tags

    HIS-HA-CMYC

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIRES2-EGFP-p53 WT

    Plasmid

    #49242

    Purpose

    expresses human wt p53 and EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. H6GB1tevP53SS(1-393)

    Plasmid

    #200311

    Purpose

    Bacterial expression of human superstable p53 as His6GB1tev fusion

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-Neo-Bam p53 V143A

    Plasmid

    #16435

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 V143A (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V143A. Mutant cDNA from human colorectal tumor CX…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

31. EF1a-hP53DD

    Plasmid

    #214080

    Purpose

    Mammalian expression of human P53DD

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    hP53DD (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. T7-p53DD-pcDNA3

    Plasmid

    #25989

    Depositor

    William Kaelin

    Article

    Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

    Insert

    tumor protein p53 (TP53 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    truncated cDNA encodes truncated protein (aa 300-…

    Promoter

    Available since

    Oct. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PX459-TP53-exon4

    Plasmid

    #217455

    Purpose

    For TP53 knockout targeting exon 4 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PX459-TP53-exon5

    Plasmid

    #217456

    Purpose

    For TP53 knockout targeting exon 5 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDESTN-MTp-p53-Venus

    Plasmid

    #53736

    Purpose

    Expresses Venus tagged p53 under the metallothionein promoter

    Depositor

    Galit Lahav

    Article

    Batchelor et al Mol Cell. 2008 May 9;30(3):277-89. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    metallothionein promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TERT

    Plasmid

    #156078

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    TERT (TERT Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGMβ1

    Plasmid

    #216202

    Purpose

    Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.

    Depositor

    Yasuko Sasaki

    Article

    Iwamoto et al Biosci Microbiota Food Health. 2022;41(1

    Type

    Empty backbone

    Use

    Shuttle vector, conjugal plasmid, theta type rep…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…

    Available since

    Jan. 21, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pHage2-EF1a-TERT K78E-IRES-Puro

    Plasmid

    #182591

    Purpose

    expresses TERT K78E in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    K78E

    Promoter

    EF-1a

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. NSK021 CMV-TO-p53R248Q (FLP-IN)

    Plasmid

    #191167

    Purpose

    Expression of human p53 mutant R248Q in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    p53(R248Q) (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R248Q

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TFORF0625

    Plasmid

    #142278

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MYSM1 (MYSM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge