We narrowed to 3,043 results for: beta-actin
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Plasmid#13452DepositorExpressionBacterialMutationnoneAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only
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pBetaActin-mNeonGreen-CAMSAP3
Plasmid#191322PurposeExpresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flowDepositorInsertmNeonGreen-CAMSAP3 (Camsap3 Mouse)
ExpressionMammalianAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/nV5-DEST-beta catenin
Plasmid#20140DepositorInsertpcDNA3.1/nV5-DEST-beta catenin (Ctnnb1 Mouse)
TagsV5ExpressionMammalianMutationT3IPromoterCMVAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NLS-actin
Plasmid#118380PurposeGateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.DepositorAvailable SinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-R62D
Plasmid#118379PurposeGateway compatible donor vector with actin-R62D.DepositorAvailable SinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-Halo-CAMSAP3
Plasmid#191329PurposeExpresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flowDepositorInsertHalo-CAMSAP3 (Camsap3 Mouse)
ExpressionMammalianAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmKikGR Actin
Plasmid#105315PurposePhotoconvertable cytoskeletal protein actin to study kinetics or dynamicsDepositorAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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HA-GFP1-10-actin-pcDNA4-TO
Plasmid#118370PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.DepositorAvailable SinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-V159N
Plasmid#118383PurposeGateway compatible donor vector with actin-V159N.DepositorAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAP Z alpha 1 beta 1 CP
Plasmid#13451DepositorAvailable SinceDec. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Vim/Actb5UTR-renilla
Plasmid#38238DepositorUseLuciferaseExpressionMammalianMutationActb promoter (1000 nt upstream) and 5' UTR …PromoterEndogenous VimAvailable SinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mScarlet
Plasmid#191324PurposeExpresses fluorescent labeled beta Tubulin to measure microtubule densityDepositorInsertTUBB2a-mScarlet (Tubb2a Mouse)
ExpressionMammalianAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mNeonGreen
Plasmid#191323PurposeExpresses fluorescent labeled beta Tubulin to measure microtubule densityDepositorInsertTUBB2a-mNeonGreen (Tubb2a Mouse)
ExpressionMammalianAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-G168D,Y169D
Plasmid#118384PurposeGateway compatible donor vector with actin-G168D,Y169D.DepositorAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_DRS-1 sgRNA / hSpCas9
Plasmid#172841PurposeMammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertDRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-Nrxn1beta AS4(-)
Plasmid#59409PurposeExpression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]DepositorAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-Dync1h1motor
Plasmid#191330PurposeExpresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
ExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644Available SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-dCer3-C2-FRB
Plasmid#191327PurposeExpresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-C2-dCer3
ExpressionMammalianAvailable SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-Dync1h1motor
Plasmid#191332PurposeExpresses the dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-Dync1h1motor (Dync1h1 Mouse)
ExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644Available SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-dCer3-C2-dCer3
Plasmid#191328PurposeExpresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membraneDepositorInsertdCer3-C2-dCer3
ExpressionMammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mEos3.2
Plasmid#191320PurposeExpresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV lightDepositorInsertTUBB2a-mEos3.2 (Tubb2a Mouse)
ExpressionMammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-IC2N-dCer3
Plasmid#191325PurposeChronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)DepositorAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-Dronpa
Plasmid#191321PurposeExpresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV lightDepositorInsertTUBB2a-Dronpa (Tubb2a Mouse)
ExpressionMammalianAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-iCre-P2A-tdTomato-T2A-Puro
Plasmid#72255PurposeExpress iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoterDepositorInsertiCre-P2A-tdTomato-T2A-Puro
UseLentiviralExpressionMammalianPromoterCB and chicken beta-actin + CMV enhancerAvailable SinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2
Plasmid#191338PurposeExpresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice culturesDepositorInsertTUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
ExpressionMammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-KIF13aMotor-mScarlet-FKBP
Plasmid#191337PurposeExpresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-KIFC1motor
Plasmid#191334PurposeExpresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-KIFC1 (KIFC1 Human)
ExpressionMammalianMutationmotor domain aa 125-673; N593KAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-KIF1aMotor-mScarlet-FKBP
Plasmid#191336PurposeExpresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-Halo-Dync1h1motor
Plasmid#191333PurposeExpresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-Halo-Dync1h1motor (Dync1h1 Mouse)
ExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644Available SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FRB-dCer3-CAAX
Plasmid#191326PurposeExpresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-dCer-CAAX
ExpressionMammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-KIFC1motorN593K
Plasmid#191335PurposeExpresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-KIFC1N593K (KIFC1 Human)
ExpressionMammalianMutationmotor domain aa 125-673; N593KAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T
Plasmid#191331PurposeExpresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
ExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644Available SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cre-IRES2-GFP
Plasmid#26646PurposeMammalian coexpression of nls-Cre and EGFP from the CAG promoterDepositorInsertCre
UseCre/LoxTagsGFP and IRES2ExpressionMammalianPromoterCAGAvailable SinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP
Plasmid#121448PurposeTo make a stable human recombinant cell line; see right thumbnail map image for PQR annotationsDepositorAvailable SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pet 3d beta tentacle delete CP
Plasmid#13300DepositorInsertcapping protein alpha 1 beta 1 (deletion 28 amino acids ) (CAPZB Chicken)
ExpressionBacterialMutationCP alpha subunit is intact with a mutated beta C-…Available SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX330_TUBA1B sgRNA / hSpCas9
Plasmid#172834PurposeMammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i4 sgRNA / hSpCas9
Plasmid#172828PurposeMammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-GFP11-Actin
Plasmid#181966PurposeHuman beta actin tagged with GFP11.DepositorAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.iGluSnFR3.v857.SGZ
Plasmid#178334PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV CAG iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2CreERT2
Plasmid#13777PurposeMammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)DepositorInsertERT2-Cre-ERT2 fusion protein
UseCre/LoxExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pX330_Actb sgRNA / hSpCas9
Plasmid#172832PurposeMammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_TOMM20 sgRNA / hSpCas9
Plasmid#172836PurposeMammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMADM-beta
Plasmid#36891DepositorInsertsBeta-Geo
thymidine kinase
ExpressionMammalianPromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cre
Plasmid#13775PurposeMammalian expression of Cre recombinase from the broadly active CAG promoter/enhancerDepositorInsertCre
UseCre/LoxTagsMycExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cre:GFP
Plasmid#13776PurposeMammalian expression of Cre recombinase fused to GFPDepositorInsertCre-GFP fusion protein
UseCre/LoxTagsGFPExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CreERT2
Plasmid#14797PurposeMammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)DepositorInsertCre-ERT2 fusion protein
UseCre/LoxExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pX330_CALR sgRNA / hSpCas9
Plasmid#172838PurposeMammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9_GFP
Plasmid#44719PurposeCo-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianPromoterCAGAvailable SinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only