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We narrowed to 820 results for: tev cleavage

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Showing: 151 - 200 of 820 results

  1. pGEX2T-TEV CHMP1B 4-199 L188A/L192A

    Plasmid
    #108289
    Purpose
    Expresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Available Since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108289-plasmid-map-sequence-id-245274

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  2. pGEX2T-TEV CHMP1B 4-199 L192A/L195A

    Plasmid
    #108291
    Purpose
    Expresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 192 to A, changed L 195 to A
    Available Since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108291-plasmid-map-sequence-id-245276

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  3. pGEX2T-TEV CHMP1B 186-199 L188A/L192A

    Plasmid
    #108288
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Available Since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108288-plasmid-map-sequence-id-245273

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  4. pGEX2T-TEV CHMP1B 186-199 L192A/L195A

    Plasmid
    #108290
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Available Since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108290-plasmid-map-sequence-id-245275

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  5. pDNA2.0 6H-FLAG-TEV-KRAS G12C

    Plasmid
    #111848
    Purpose
    Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12C (KRAS Human)
    Tags
    6xHis-tag, FLAG-tag, and TEV protease cleavage se…
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Cysteine
    Promoter
    T5
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111848-plasmid-map-sequence-id-213454

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  10. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Article
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Promoter
    T7 lac promoter
    Available Since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169168-plasmid-map-sequence-id-332048

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  11. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid
    #111903
    Purpose
    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.
    Depositor
    Andrew Carter
    Inserts
    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    Tags
    8xHis, SNAPf-tag, TEV site, and ZZ-tag
    Expression
    Insect
    Mutation
    Optimised for expression in Sf9 cells.
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111903-plasmid-map-sequence-id-215307

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  12. CPH3566_GST-TEV-Spt6p-tSH2-1223-1451

    Plasmid
    #227085
    Purpose
    Bacterial expression of GST-tagged Spt6 tSH2 domain (1223-1451)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227085-plasmid-map-sequence-id-451055

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  13. CPH3737_GST-TEV-Spt6p-tSH2-1223-1453

    Plasmid
    #227087
    Purpose
    Bacterial expression of K1355A/K1435A double-mutant GST-tagged Spt6 tSH2 domain (1223-1453)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    K1355A, K1435A
    Promoter
    T7
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    227087-plasmid-map-sequence-id-470399

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  14. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

    Plasmid
    #227086
    Purpose
    Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227086-plasmid-map-sequence-id-451101

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  15. CPH3736_GST-TEV-Spt6p-tSH2-1223-1454

    Plasmid
    #227088
    Purpose
    Bacterial expression of R1282H/K1355A/K1435A triple-mutant GST-tagged Spt6 tSH2 domain (1223-1454)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H, K1355A, K1435A
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227088-plasmid-map-sequence-id-451126

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  16. CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268

    Plasmid
    #227089
    Purpose
    Bacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)
    Depositor
    Chris Hill
    Insert
    tom1p-HECT (TOM1 Budding Yeast)
    Tags
    HIS-MBP-TEV_cleavage_site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227089-plasmid-map-sequence-id-451122

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  17. pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG

    Plasmid
    #217324
    Purpose
    Purification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S969A
    Promoter
    polyhedrin
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217324-plasmid-map-sequence-id-438435

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  19. pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

    Plasmid
    #217321
    Purpose
    Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S969A
    Promoter
    polyhedrin
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217321-plasmid-map-sequence-id-433443

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  20. pET28-MKH8SUMO

    Plasmid
    #79526
    Purpose
    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    8xHis-Thrombin-SUMO-TEV and TEV cleavage site
    Expression
    Bacterial
    Available Since
    June 16, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. 371-B

    Plasmid
    #97027
    Purpose
    His6-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97027-plasmid-map-sequence-id-182504

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  22. 371-GFP

    Plasmid
    #97030
    Purpose
    His6-GFP-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    GFP fusion, His6 tag, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97030-plasmid-map-sequence-id-182506

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  23. 371-Bc

    Plasmid
    #97028
    Purpose
    yORF-tev-His6
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97028-plasmid-map-sequence-id-182499

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  24. 371-T

    Plasmid
    #97035
    Purpose
    His6-Thioredoxin-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag, TEV cleavage site, and Thioredoxin funs…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97035-plasmid-map-sequence-id-182503

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  25. 371-R

    Plasmid
    #97033
    Purpose
    StrepII-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    StrepII tag and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97033-plasmid-map-sequence-id-182501

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  26. 371-S

    Plasmid
    #97034
    Purpose
    His6-Sumo-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag, SUMO domain, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97034-plasmid-map-sequence-id-182498

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  27. 371-O

    Plasmid
    #97032
    Purpose
    His6-Mocr-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag, TEV cleavage site, and mOCR fusion
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97032-plasmid-map-sequence-id-182497

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  28. 371-L

    Plasmid
    #97031
    Purpose
    FLAG-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    FLAG tag and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97031-plasmid-map-sequence-id-182492

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  29. 371-C

    Plasmid
    #97029
    Purpose
    His6-MBP-N10-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag, MBP domain, N10 linker, and TEV cleavag…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97029-plasmid-map-sequence-id-182500

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  31. pAAP2

    Plasmid
    #171208
    Purpose
    bacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV protease
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    pgl-3 (pgl-3 Nematode)
    Tags
    MBP::HIS::TEV cleavage site
    Expression
    Bacterial
    Available Since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171208-plasmid-map-sequence-id-335799

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  32. pJK635

    Plasmid
    #72473
    Purpose
    pET28a modified to contain TEV protease fusion tag instead of T7 tag
    Depositor
    T. J. Kappock
    Type
    Empty backbone
    Tags
    His6 and TEV protease cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72473-plasmid-map-sequence-id-132656

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  33. pst39-NST-clone14

    Plasmid
    #133421
    Purpose
    S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).
    Depositor
    Gerhard Wagner
    Insert
    NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
    Expression
    Bacterial
    Mutation
    ORF for Nip1 encodes aa 4-156 (not complete ORF),…
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133421-plasmid-map-sequence-id-267716

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  35. pQE80l B-CC-B

    Plasmid
    #196479
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCC
    Depositor
    Fei Sun
    Insert
    B-CC-B
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196479-plasmid-map-sequence-id-387244

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  36. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Promoter
    T7-lac0
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

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  37. pNIC28-Bsa4-iLOVf

    Plasmid
    #63722
    Purpose
    LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Tags
    TEE, iLOV, 6xHis, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63722-plasmid-map-sequence-id-106345

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  39. pET-28a_VIM-2

    Plasmid
    #242840
    Purpose
    Expresses His6-TEV-VIM-2 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-2 sequence (UniPROT Q5U7L7)
    Depositor
    Richard Page
    Article
    Silwal et al J Inorg Biochem. 2025 Oct;271:112975. do
    Insert
    Metallo-beta-lactamase VIM-2
    Tags
    His6 + Tobacco etch virus (TEV) cleavage site “EN…
    Expression
    Bacterial
    Mutation
    deleted residues 1-26
    Promoter
    T7
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242840-plasmid-map-sequence-id-486287

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  40. pET-28a_VIM-20

    Plasmid
    #242842
    Purpose
    Expresses His6-TEV-VIM-20 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-20 sequence (UniPROT A0A344X7M2)
    Depositor
    Richard Page
    Article
    Silwal et al J Inorg Biochem. 2025 Oct;271:112975. do
    Insert
    Metallo-beta-lactamase VIM-20
    Tags
    His6 + Tobacco etch virus (TEV) cleavage site “EN…
    Expression
    Bacterial
    Mutation
    deleted residues 1-26
    Promoter
    T7
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242842-plasmid-map-sequence-id-486284

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  41. pET-28a_VIM-15

    Plasmid
    #242841
    Purpose
    Expresses His6-TEV-VIM-15 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-15 sequence (UniPROT B4YAJ6)
    Depositor
    Richard Page
    Article
    Silwal et al J Inorg Biochem. 2025 Oct;271:112975. do
    Insert
    Metallo-beta-lactamase VIM-15
    Tags
    His6 + Tobacco etch virus (TEV) cleavage site “EN…
    Expression
    Bacterial
    Mutation
    deleted residues 1-26
    Promoter
    T7
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242841-plasmid-map-sequence-id-486280

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  42. pET-28a_VIM-31

    Plasmid
    #242843
    Purpose
    Expresses His6-TEV-VIM-31 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-31 sequence (UniPROT I3RJZ3)
    Depositor
    Richard Page
    Article
    Silwal et al J Inorg Biochem. 2025 Oct;271:112975. do
    Insert
    Metallo-beta-lactamase VIM-31
    Tags
    His6 + Tobacco etch virus (TEV) cleavage site “EN…
    Expression
    Bacterial
    Mutation
    deleted residues 1-26
    Promoter
    T7
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242843-plasmid-map-sequence-id-486281

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  43. thoc5.018.389

    Plasmid
    #137266
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    thoc5 (THOC5 Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    018-389
    Available Since
    Feb. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    137266-plasmid-map-sequence-id-323966

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  44. NKAP.001.140

    Plasmid
    #137206
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    NKAP (NKAP Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-140
    Available Since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    137206-plasmid-map-sequence-id-322594

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  46. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid
    #86928
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and td…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    86928-plasmid-map-sequence-id-175058

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  47. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

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  48. pET mRuby2 LIC cloning vector (H6-mRuby2)

    Plasmid
    #86934
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and mR…
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86934-plasmid-map-sequence-id-174750

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  49. pFB-CT10HF-LIC

    Plasmid
    #39191
    Purpose
    SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    TEV-His-FLAG
    Promoter
    polyhedrin
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39191-plasmid-map-sequence-id-136169

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  50. pET mClover LIC cloning vector (H6-mClover)

    Plasmid
    #86931
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-mClover
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and mC…
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86931-plasmid-map-sequence-id-174751

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