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Expression_vector_RO2_9

Plasmid

#158018

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_9

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_9

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Expression_vector_RO2_10

Plasmid

#158019

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_10

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_10

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_15

Plasmid

#158020

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_15

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_15

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_20

Plasmid

#158021

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_20

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_20

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_25

Plasmid

#158022

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_25

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_25

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_NT_1

Plasmid

#158023

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_1

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_1

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_NT_8

Plasmid

#158024

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_8

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_8

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_NT_9

Plasmid

#158025

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_9

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_9

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_NT_10

Plasmid

#158026

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_10

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_10

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL1777 (pEffector)

Plasmid

#160731

Purpose

Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins and crRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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peYFP-C1-mTOR

Plasmid

#73384

Purpose

Expresses YFP tagged mTOR

Depositor

Taekjip Ha, Jie Chen

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

mTOR (MTOR Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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mTOR_2 shRNA

Plasmid

#1856

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

mTOR (MTOR Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
Factor IX donor

Plasmid

#182141

Purpose

Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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EFS-Vector

Plasmid

#109395

Purpose

AAV-CRISPR knockout plasmid for direct in vivo genome editing

Depositor

Sidi Chen

Article

Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Tags

Expression

Mammalian

Mutation

Promoter

EFs-NS

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SAMTOR

Plasmid

#100513

Purpose

Expresses HA tagged SAMTOR in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pZW1-snoVector

Plasmid

#73174

Purpose

Nuclear expression of RNAs

Depositor

Ling-Ling Chen

Article

Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

Inserts

SNORD116-13 (SNORD116-13 Human)
SNORD116-14 (SNORD116-14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pC1-mitoRexYFP

Plasmid

#60246

Purpose

Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

Depositor

Vsevolod Belousov

Article

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

mitoRexYFP

Use

Tags

Mitochondrial targeting signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Rictor_1 shRNA

Plasmid

#1853

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

Rictor (RICTOR Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2005

Availability

Academic Institutions and Nonprofits only

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myc-mTOR

Plasmid

#1861

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

mTOR (Mtor Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge