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lentiCRISPRv2_AMBRA1#2

Plasmid

#174153

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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ptdTHC_PGKneoLox2DTA.2

Plasmid

#112625

Purpose

H2BtdTomato_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-tdTomato (H2BC3 Human, Anthozoa)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

tdTomato

Promoter

No Promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pVHC_PGKneoLox2DTA.2

Plasmid

#112622

Purpose

H2BVenus_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Venus (H2BC3 Aequorea victoria, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

Venus

Promoter

No Promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgAMBRA1#2

Plasmid

#174147

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pmTHC_PGKneoLox2DTA.2

Plasmid

#112624

Purpose

H2BmTeal_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-mTFP1 (H2BC3 Human, Anthozoa)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

mTeal (mTFP1)

Promoter

No Promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEHC_PGKneoLox2DTA.2

Plasmid

#112623

Purpose

H2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Emerald (H2BC3 Aequorea victoria, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

Emerald

Promoter

No Promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA#2

Plasmid

#64246

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A-T#2

Plasmid

#171516

Purpose

deletion of a genomic locus in T(Brachyury) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

T (T Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Cilantro 2

Plasmid

#74450

Purpose

Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pconst #2-RFP

Plasmid

#81076

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter- BBa_J23104

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP670-2

Plasmid

#197238

Purpose

Expresses the fusion protein of Tubulin-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP670-2

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pmOrange 2-Actin

Plasmid

#199703

Purpose

visualization of cleavage furrow in living mammalian mitotic cells

Depositor

Kenji Sugimoto

Article

Tanaka et al Bioimages 25: 7-19, 2017

Insert

human cytoplasmic action

Tags

mOrange 2

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgAP2S1 #2

Plasmid

#202757

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting AP2S1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9-sgAAVS1-2

Plasmid

#129727

Purpose

Expressing SpCas9 and sgAAVS1-2

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

SpCas9 and sgAAVS1-2

Use

CRISPR

Tags

T2A-mCherry-P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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RUBY-ABE#2

Plasmid

#232176

Purpose

T-DNA vector with ecTadA8e-zCas9D10A and corresponding sgRNA to correct RUBYm2 and recover a vivid red color visible to the naked eyes.

Depositor

Yiping Qi

Article

Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265

Insert

2x35s-ecTadA8e-SpCas9-D10A-AtU3-sgRNA-mis4-gRNA scaffold

Use

CRISPR

Tags

3xflag and NLS

Expression

Plant

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2)

Plasmid

#241056

Purpose

Expresses SARS-CoV-2 spike (BA.2) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,V213G,G339D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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NO- DSD Sensor 2

Plasmid

#199786

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO- DSD Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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HUWE1-sgRNA-2

Plasmid

#86925

Purpose

To express sgRNA target HUWE1 gene

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

HUWE1 sgRNA2 (HUWE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only
AAV.CBA.YFP.miR-E_shSrpk3-2

Plasmid

#180401

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-2 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-2

Plasmid

#180392

Purpose

Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-2 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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