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We narrowed to 136 results for: tracrRNA

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Showing: 61 - 80 of 136 results

  1. UC20m

    Plasmid
    #121040
    Purpose
    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
    Depositor
    Richard Murray
    Insert
    gN2 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121040-plasmid-map-sequence-id-236353

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  2. pLY305

    Plasmid
    #184159
    Purpose
    Reporter circuit for hijacking mRNA of zraP as CRISPR RNA
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    mutsfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    K140N, Mutations D10A and H840A on WT cas9. Synon…
    Promoter
    Pcon, PpspA-zraP-22, PrhaB, and Ptet
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184159-plasmid-map-sequence-id-376083

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  3. pLY315

    Plasmid
    #184160
    Purpose
    Reporter circuit for hijacking mRNA of zntA as CRISPR RNA
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-zntA-1034, PrhaB, and Ptet
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    184160-plasmid-map-sequence-id-376259

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  4. pLY300

    Plasmid
    #184158
    Purpose
    Reporter circuit for hijacking small RNA oxyS as CRISPR RNA
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-oxyS-86, PrhaB, and Ptet
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184158-plasmid-map-sequence-id-376084

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  5. pLY320

    Plasmid
    #184161
    Purpose
    Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-cusCFAB-656, PrhaB, and Ptet
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184161-plasmid-map-sequence-id-376082

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  6. pLY164

    Plasmid
    #184140
    Purpose
    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-R3, PrhaB, and Ptet
    Available Since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184140-plasmid-map-sequence-id-378329

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  7. pLY162

    Plasmid
    #184138
    Purpose
    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-R1, PrhaB, and Ptet
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184138-plasmid-map-sequence-id-376257

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  8. pLY163

    Plasmid
    #184141
    Purpose
    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-R2, PrhaB, and Ptet
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184141-plasmid-map-sequence-id-376139

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  9. pLY168

    Plasmid
    #184142
    Purpose
    Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-Ar2, PrhaB, and Ptet
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184142-plasmid-map-sequence-id-376136

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  12. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

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  15. pWJ66

    Plasmid
    #46570
    Purpose
    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9-w
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46570-plasmid-map-sequence-id-65846

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  18. pSM-dCas9::Ap

    Plasmid
    #154307
    Purpose
    Shuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    I-Son Ng
    Article
    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154307-plasmid-map-sequence-id-306378

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Showing: 61 - 80 of 136 results