We narrowed to 5,620 results for: pcas

1. pCAG-nDsRedFL-intron

   Plasmid

   #177804

   Purpose

   An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   intron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. hGli3 NTOTAL in AINGpCAGGS

   Plasmid

   #121973

   Purpose

   medium Gli3 transcriptional repressor

   Depositor

   James Briscoe

   Article

   Ulloa et al Curr Biol. 2007 Mar 20;17(6):545-50. doi

   Insert

   hGli3 NTOTAL (GLI3 Human)
   

   Use

   Chick electroporation

   Tags

   5xmyc and Linker A IRES NLS GFP pCAGGS

   Expression

   Mammalian

   Mutation

   C terminus deletion from zinc finger domain

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-GFP-G3BP1(2)

   Plasmid

   #136059

   Purpose

   G3BP1 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTATTACACACTGCTGAACC)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAS9-mCherry-TUBB

   Plasmid

   #66942

   Purpose

   CRISPaint target selector TUBB

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA TUBB
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-p65AD-GBP6

   Plasmid

   #49440

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP6-p65 transactivation domain fusion protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGIG-6xHIS.Mm.RPE65-eGFP

   Plasmid

   #41019

   Depositor

   John Nickerson

   Insert

   RPE65 (Rpe65 Mouse)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-GST FNIP2

   Plasmid

   #164982

   Purpose

   FNIP2 expressing vector for mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FNIP2 (FNIP2 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-PSD95-GCaMP2

   Plasmid

   #18931

   Depositor

   Karel Svoboda

   Article

   Mao et al PLoS ONE. 2008 . 3(3):e1796.

   Insert

   PSD95-GCaMP2 (Dlg4 Rat)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pFREE2-SpCas9-sgRNA

   Plasmid

   #179582

   Purpose

   Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-HA-NLGN3 R451C

    Plasmid

    #59319

    Purpose

    Expression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutation

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN3 R451C (NLGN3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Arginine 451 to Cysteine (R451C mutation)

    Promoter

    CAG

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-GFP-G3BP1(1)

    Plasmid

    #136058

    Purpose

    G3BP1 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTAGTCCCCTGCTGGTCGGGC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG MCP-VP64

    Plasmid

    #92357

    Purpose

    CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    MCP-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-H2B-GFP

    Plasmid

    #184777

    Purpose

    Expresses H2B-GFP in human pluripotent stem cells

    Depositor

    Ivana Barbaric

    Article

    Price et al Curr Protoc. 2022 May;2(5):e435. doi: 10

    Insert

    H2BC11 (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DN2-Gli3 in AINGpCAGGS

    Plasmid

    #121969

    Purpose

    strong Gli3 transcriptional activator

    Depositor

    James Briscoe

    Article

    Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

    Insert

    DN2Gli3 (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus deletion (nt1-1401)

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-sREACh-Actin

    Plasmid

    #21949

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

    Insert

    betaActin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pCAGGS-4EBP1 F113A

    Plasmid

    #81122

    Purpose

    expresses a constitutively active mutant of 4E-BP1 under the CAG promoter

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    4EBP1 F113A (Eif4ebp1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F113A mutation

    Promoter

    CAG

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-AsCas12a-2AeGFP

    Plasmid

    #123638

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    AsCas12a
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-Neo-PuroDTK

    Plasmid

    #84036

    Purpose

    PiggyBac cassette contains Neomycin, puromycin and TK genes

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330_TOMM20 sgRNA / hSpCas9

    Plasmid

    #172836

    Purpose

    Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-mDrc7-FLAG

    Plasmid

    #176470

    Purpose

    Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    dynein regulatory complex subunit 7 (Drc7 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

    Plasmid

    #107726

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-Venus-MDGA1

    Plasmid

    #82543

    Purpose

    Overexpression vector for Venus-MDGA1 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    MDGA1 (MDGA1 Human)
    

    Use

    Tags

    Venus fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta actin

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-rTetRDBD-GBP1

    Plasmid

    #50791

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    rTetR DNA binding domain-GBP1 fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-AaCas12b-2AeGFP

    Plasmid

    #121945

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAFNF-ECFP-Actb

    Plasmid

    #169787

    Purpose

    An actin FRET sensor. Must be expressed with Venus-actin for FRET.

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Actb (Actb Mouse)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-cofilin S3A

    Plasmid

    #163604

    Purpose

    Expresses constitutively active cofilin in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    cofilin1 (Cfl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S3A substitution to make cofilin constitutively a…

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-Ace2_D92N_E199V-mRuby3

    Plasmid

    #129257

    Purpose

    Express Ace2_D92N_E199V-mRuby3 in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace2_D92N_E199V-mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available since

    Aug. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSpCas9n(BB)-2A-puro-a5 guide B

    Plasmid

    #194011

    Purpose

    For CRISPR knockout of integrin alpha5

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (a5_guide B)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSpCas9n(BB)-2A-puro-aV guide A

    Plasmid

    #194008

    Purpose

    For CRISPR knockout of integrin alphaV

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (aV_guide A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSpCas9n(BB)-2A-puro-aV guide B

    Plasmid

    #194009

    Purpose

    For CRISPR knockout of integrin alphaV

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    single-guide RNA (aV_guide B)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge