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Showing: 1561 - 1600 of 1645 results

  1. pLJM1-FIRE-pHLy

    Plasmid
    #170775
    Purpose
    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    CMV
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170775-plasmid-map-sequence-id-339023

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  2. pFUGW-FIRE-pHLy

    Plasmid
    #170774
    Purpose
    Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    hUbC
    Available since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    170774-plasmid-map-sequence-id-339008

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  10. pAAV-U6-racRNA-A

    Plasmid
    #200824
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200824-plasmid-map-sequence-id-396714

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  11. pAAV-U6-racRNA-B

    Plasmid
    #200825
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200825-plasmid-map-sequence-id-396771

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  12. pAAV-U6-racRNA-C

    Plasmid
    #200826
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200826-plasmid-map-sequence-id-396715

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  13. pAAV-U6-racRNA-D

    Plasmid
    #200827
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200827-plasmid-map-sequence-id-396773

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  20. pCKC776

    Plasmid
    #226627
    Purpose
    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    Use
    Synthetic Biology
    Tags
    oxygen dependent degron (ODD)
    Expression
    Mammalian
    Mutation
    Promoter
    4xHRE_YB TATA and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226627-plasmid-map-sequence-id-449157

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  24. pAP-U2-1

    Plasmid
    #236472
    Purpose
    Plasmid for integration at the native URA3 locus with 3xmCherry and 3xGFP under control of the strong bidirectional SpH2A/B promoter.
    Depositor
    Daniel Lew
    Article
    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.
    Insert
    spH2Ap-3xmCherry spH2Bp-3xGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for A. pullulans
    Promoter
    Available since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236472-plasmid-map-sequence-id-468916

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  25. AP2u2-CMV-Ace2-IgG1

    Plasmid
    #226455
    Purpose
    Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Inserts
    Angiotensin Converting Enzyme 2
    Immunoglobulin Heavy Constant Gamma 1
    Use
    Tags
    6xHis Tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226455-plasmid-map-sequence-id-458394

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  26. pAP-U2-3

    Plasmid
    #236474
    Purpose
    Plasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApACT1 promoter.
    Depositor
    Daniel Lew
    Article
    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.
    Insert
    scACT1p-3xmCherry apACT1p-3xGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for A. pullulans
    Promoter
    Available since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236474-plasmid-map-sequence-id-468914

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  27. pAP-U2-4

    Plasmid
    #236475
    Purpose
    Plasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApTUB1 promoter.
    Depositor
    Daniel Lew
    Article
    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.
    Insert
    scACT1p-3xmCherry apTUB1p-3xGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for A. pullulans
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236475-plasmid-map-sequence-id-468488

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  28. pAP-U2-2

    Plasmid
    #236473
    Purpose
    Plasmid for integration at the native URA3 locus with 3xmCherry under control of the SpH2B promoter and 3xGFP under control of the ScACT1 promoter.
    Depositor
    Daniel Lew
    Article
    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.
    Insert
    spH2Bp-3xmCherry scACT1p-3xGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for A. pullulans
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236473-plasmid-map-sequence-id-468487

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  29. pTuLys2CMR2

    Plasmid
    #53544
    Purpose
    Inhibition module. Constructed from the parent plasmids pLuxRI, pLuxmCherry, pET15bLCFPT7, and pLysS. The plasmid can express T7 lysozyme by inducing AHL and LuxR. Chloramphenicol resistance
    Depositor
    Lingchong You
    Article
    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10
    Insert
    pLux
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    53544-plasmid-map-sequence-id-83437

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  34. pCherryGFP-CoV2-Inframe-stop

    Plasmid
    #177620
    Purpose
    Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).
    Depositor
    Junjie Guo
    Article
    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11
    Insert
    SARS-CoV-2 FSE
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177620-plasmid-map-sequence-id-350046

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  35. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

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  36. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid
    #134923
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134923-plasmid-map-sequence-id-267593

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Showing: 1561 - 1600 of 1645 results