We narrowed to 22,194 results for: tes

1. actc1b-2PH-PLCd-mKate2

   Plasmid

   #109500

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-2PH-PLCd-mKate2
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GA-NES

   Plasmid

   #61018

   Purpose

   ddGFP A-NES

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP A
   

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTn7CdrA-gfp(ASV)C

   Plasmid

   #111617

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(ASV)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTn7CdrA-gfpC

   Plasmid

   #111616

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

   Plasmid

   #162333

   Purpose

   Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSpCas9-2xVP64
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPHT-PIP2

   Plasmid

   #60937

   Purpose

   Fluorescent FPX sensor for PIP2

   Depositor

   Anne Marie Quinn

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   PIP2 dimerization dependent sensor RA+B monomers
   

   Tags

   dimerization dependent fluorescent protein monome…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDN34-BiNLS-hYAP1-1delta

   Plasmid

   #169471

   Purpose

   expresses optoYAP in mammalian cells

   Depositor

   Timothy Saunders

   Article

   Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

   Insert

   YAP1-1delta (YAP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. RA-NES

   Plasmid

   #61019

   Purpose

   ddRFP A-NES

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A
   

   Tags

   HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTet-O-RUNX3-T2A-PuroR

   Plasmid

   #162349

   Purpose

   Lentiviral expression of RUNX3 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   RUNX3 (RUNX3 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Promoter

   Tet-ON

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5 FLAG metap2

    Plasmid

    #32004

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    metap2 (Metap2 )
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pME-loxP-mTagBFP2-stop-pA-loxP

    Plasmid

    #75158

    Purpose

    Floxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    loxP-mTagBFP2-stop-pA-loxP
    

    Use

    Middle entry vector for multisite gateway three f…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2p2A--3mnx1:mTagBFP-NS-MCS

    Plasmid

    #78250

    Purpose

    Can be used to subclone in gene of interest into blue motor neuron expression vector.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Inserts

    -3mnx1 (mnx1 Zebrafish)
    -mTagBFP-NS-MCS
    

    Use

    Zebrafish motor neuron expression

    Promoter

    -3mnx1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. acta1-Cavin1a-Clover

    Plasmid

    #163852

    Purpose

    Zebrafish muscle specific expression of Cavin1a-Clover, Parton lab clone DPR

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    Cavin1a (cavin1a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Clover

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3E-Rac1-DN

    Plasmid

    #109582

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative Rac1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rac1 (rac1a Zebrafish)
    

    Use

    Zebrafish plasmids

    Mutation

    dominant negative

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-Cavin4b-TurboID

    Plasmid

    #163851

    Purpose

    In vitro transcription of Cavin4b-TurboID. Parton lab clone JTA

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    Cavin4b (cavin4b Zebrafish)
    

    Use

    In vitro transcription of rna

    Tags

    TurboID

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

    Plasmid

    #83925

    Purpose

    Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    eGFP
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Promoter

    hUbC

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #162334

    Purpose

    Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSaCas9-KRAB
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. ML1Nmut_in_pEGFP-C1

    Plasmid

    #67796

    Purpose

    Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2(7Q)-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-MLC2(CA)-IRES-GFP

    Plasmid

    #133928

    Purpose

    Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    EGFP and MLC2(CA) (MYL2 Human)
    

    Expression

    Mammalian

    Mutation

    MLC2 T18D, S19D

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPBAD-yhjH

    Plasmid

    #111613

    Purpose

    Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

    Depositor

    Tim Tolker-Nielsen

    Article

    Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

    Insert

    yhjH
    

    Expression

    Bacterial

    Mutation

    A52V

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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