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173,383 results

Showing: 11971 - 12000 of 173383 results

  1. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

    Plasmid
    #134653
    Purpose
    lysosome-targeted Sac1 catalytic domain C389S
    Depositor
    Roberto Zoncu
    Article
    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218
    Insert
    Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
    Use
    Lentiviral
    Tags
    FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…
    Expression
    Mammalian
    Mutation
    C389S and contains only amino acids 77-520 (Pleas…
    Available Since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    134653-plasmid-map-sequence-id-266494

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  3. qTAG-N-Solo-mStayGold-GOLGA2

    Plasmid
    #227325
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227325-plasmid-map-sequence-id-452110

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  4. pcDNA3.1.Gα13-LgB106

    Plasmid
    #134364
    Purpose
    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα13-LgB106 (GNA13 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134364-plasmid-map-sequence-id-306388

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  12. pBV65

    Plasmid
    #108363
    Purpose
    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.
    Depositor
    Scott Moye-Rowley
    Article
    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0
    Insert
    loxP-natMX6-CgMET3prom-cre-loxP
    Use
    Gene disruption marker for candida glabrata
    Promoter
    C. glabrata MET3
    Available Since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108363-plasmid-map-sequence-id-209915

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  14. pGLO-Blue

    Plasmid
    #220782
    Purpose
    Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)
    Depositor
    Angela Leu
    Insert
    blue chromoprotein (aeBlue)
    Tags
    8xHis
    Expression
    Bacterial
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220782-plasmid-map-sequence-id-437912

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  17. dCas12Max-ABE

    Plasmid
    #195335
    Purpose
    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-ABE
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195335-plasmid-map-sequence-id-383124

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  22. pUC18 mouse rDNA R1

    Plasmid
    #240559
    Purpose
    An rDNA FISH probe contains 10.7 kb fragment that spans the 3′ end of the intergenic spacer (IGS) and the beginning of the external transcribed spacer (ETS).
    Depositor
    Brian McStay, Shan Zha
    Insert
    Partial mouse rDNA sequence
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Dec. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    240559-plasmid-map-sequence-id-485695

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Showing: 11971 - 12000 of 173383 results