We narrowed to 8,596 results for: Set

1. pAC8_MF-p10-Flag-Cter(VE5738)

   Plasmid

   #161798

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   C-terminal Flag tag
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   p10

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC8_PH-10His-Nter-GWs-Lox (VE5588)

   Plasmid

   #161806

   Purpose

   Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   N-terminal 10His tag
   

   Tags

   10 His

   Expression

   Insect

   Promoter

   PH

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC8_MF-p10-mCh PH-mCh (VE5625)

   Plasmid

   #139769

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   mCherry fluorescent protein
   

   Expression

   Insect

   Promoter

   PH or p10

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_Donor/Reporter-COL4A5

   Plasmid

   #130280

   Purpose

   COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   mCherry-eGFP
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

   Plasmid

   #119864

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Tags

   HA-tagged biotin ligase (BirA), Thosea asigna vir…

   Expression

   Bacterial

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDF-casBCDE(g8)

   Plasmid

   #89727

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO_AU1.HSV.Ollas_mCherry-NLS

   Plasmid

   #178223

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.HSV.Ollas and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO_AU1.HA.V5_mCherry-NLS

   Plasmid

   #178221

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.HA.V5 and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO_AU1.HA.Ollas_mCherry-NLS

   Plasmid

   #178218

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.HA.Ollas and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO_AU1.C.V5_mCherry-NLS

    Plasmid

    #178215

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.V5 and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO_AU1.C.Ollas_mCherry-NLS

    Plasmid

    #178212

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.Ollas and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO_HSV.Ollas.S_mCherry-NLS

    Plasmid

    #178276

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HSV.Ollas.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO_HA.HSV.Ollas_mCherry-NLS

    Plasmid

    #178259

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.HSV.Ollas and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO_HA.HSV.NWS_mCherry-NLS

    Plasmid

    #178258

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.HSV.NWS and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-PLXNB2-decto

    Plasmid

    #86238

    Purpose

    Lentivirus for expression of human Plexin-B2 with deletion of ectodomain

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    PLXNB2 with deletion of extracellular domain

    Promoter

    hPGK

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-PLXNB2-mRBD

    Plasmid

    #86240

    Purpose

    Lentivirus for expression of human Plexin-B2 with mutant RBD domain

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    PLXNB2 with point mutations of RBD domain

    Promoter

    hPGK

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-PLXNB2-dVTDL

    Plasmid

    #86239

    Purpose

    Lentivirus for expression of human Plexin-B2 with deletion of C-terminal VTDL aminoacids

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    PLXNB2 with deletion of last 4 aminoacids

    Promoter

    hPGK

    Available Since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

    Plasmid

    #154779

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    LaG17-SynNotch
    

    Tags

    LaG17

    Expression

    Mammalian

    Mutation

    204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3-HPRT1-S104R-PdTK-mCh

    Plasmid

    #107276

    Purpose

    HPRT1 c.312C>A Munich donor vector with unilateral microhomology

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Inserts

    HPRT genomic DNA (HPRT1 Human)
    puro-deltaTK; CAG::mCherry
    

    Mutation

    c.306G>T, c.312C>A

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRT2-H2B-EGFP-V5-2A (IG157)

    Plasmid

    #99623

    Purpose

    To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    H2B-EGFP-V5
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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