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pCDH-puro-EWS-FLI1

Plasmid

#102813

Purpose

Overexpress EWS-FLI1. 3rd generation lentiviral vector.

Depositor

Jialiang Wang

Article

Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

Insert

EWS-FLI1 (EWSR1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Expression

Bacterial

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pEF6-Acly

Plasmid

#70765

Purpose

Expresses mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Article

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Tags

myc-epitope

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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Murine E-cadherin mCherry

Plasmid

#71366

Purpose

mammalian expression of murine E-caderin with mCherry fusion

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

E-cadherin (Cdh1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pmIL-6 FL

Plasmid

#61286

Purpose

drives luciferase from Wt mouse IL-6 promoter (full length)

Depositor

Gail Bishop

Article

Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

Insert

IL-6 promoter (Il6 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

IL-6 full length promoter

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pBAMD1-2

Plasmid

#61564

Purpose

Standardized mini-Tn5 delivery plasmid, kanamycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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T7-VEE-OKS-iG

Plasmid

#58974

Purpose

RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Inserts

Oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
GLIS (GLIS1 Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-H2B-GCaMP6s

Plasmid

#59530

Purpose

Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
H2B
GCaMP6s

Use

Transposable element

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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