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Showing: 8081 - 8100 of 65925 results

  5. pAH-CTX1-rhadCas9

    Plasmid
    #129391
    Purpose
    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
    Depositor
    Silvia Cardona
    Publication
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Burkholderia cenocepacia codon-optimized dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Entire dCas9 codon optimized for the GC-rich B. c…
    Promoter
    PrhaBAD
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    129391-plasmid-map-sequence-id-273754

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  12. pFD116

    Plasmid
    #124769
    Purpose
    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Publication
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Ptet
    dcas9
    PpflB sgRNA BsaI
    Use
    CRISPR
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124769-plasmid-map-sequence-id-246181

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  13. pcDNA3.1-CD40L-mgSrtA

    Plasmid
    #125795
    Purpose
    for co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.
    Depositor
    P. Chen
    Publication
    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD40L-mgSrtA
    Expression
    Mammalian
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    125795-plasmid-map-sequence-id-245283

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  19. pEUI(+)

    Plasmid
    #111834
    Purpose
    For mamalian expression of Ecdysone receptor-based inducible transgene expression switch.
    Depositor
    Hyunju Ro
    Publication
    Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAL4-minimal VP16-EcR (EcR Synthetic)
    Expression
    Mammalian
    Promoter
    CMV, SV40, 10XUAS
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    111834-plasmid-map-sequence-id-218213

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  20. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    100936-plasmid-map-sequence-id-206124

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Showing: 8081 - 8100 of 65925 results