We narrowed to 10,508 results for: ADA

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pPB-AMBRA1-3xFLAG

Plasmid

#174159

Purpose

PiggyBac vector to stably express 3xFLAG-tagged AMBRA1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1A

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-tdTHC

Plasmid

#112621

Purpose

expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-tdTomato (H2BC3 Anthozoa, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox

Tags

tdTomato

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 N C-V5-tag

Plasmid

#179968

Purpose

Mammalian expression vector for SARS-CoV-1 N, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 N (N Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP3 C-V5-tag

Plasmid

#179962

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp3, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 Nsp3 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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8798 GFP-KrasG12C_2B_hygro

Plasmid

#64376

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

changed glycine 12 to cysteine

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 ORF3a C-V5-tag

Plasmid

#179988

Purpose

Mammalian expression vector for RaTG13 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

RaTG13 ORF3a

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ptdTHC_PGKneoLox2DTA.2

Plasmid

#112625

Purpose

H2BtdTomato_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-tdTomato (H2BC3 Anthozoa, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

tdTomato

Promoter

No Promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 NSP15 C-V5-tag

Plasmid

#179975

Purpose

Mammalian expression vector for SARS-CoV-2 Nsp15, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-2 Nsp15 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP G55A in Artichoke

Plasmid

#169935

Purpose

Overexpression of BCL6-GFP G55A mutant fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 G55A (BCL6 Human)

Use

Lentiviral

Tags

GFP

Mutation

160R>C

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-Myc-FBW7 Delta F-Box

Plasmid

#197452

Purpose

The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW7 Fbox deleted.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2_shB3GNT5.1

Plasmid

#110325

Purpose

TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

B3GNT5 (B3GNT5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6

Plasmid

#180313

Purpose

bacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN6 (SEPTIN6 Human)

Tags

His6-TEV and sfCherry2

Expression

Bacterial

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-hCCND1-T286A-HA

Plasmid

#174155

Purpose

Lentiviral vector expressing HA-tagged human cyclin D1 with T286A mutation

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

hemagglutinin (HA)

Expression

Mammalian

Mutation

A>G at base 856 (Thr>Ala at amino acid 286)

Promoter

EF1A

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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8047_GFP-Kras2B_ires_puro

Plasmid

#64371

Purpose

This a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

none

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-V5-SHLD3

Plasmid

#114124

Purpose

Expresses N-terminally tagged V5-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

V5

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 23, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DHC1-AID-Clover donor (Neo)

Plasmid

#158621

Purpose

Donor plasmid for tagging DHC1 with AID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR; Tagging donor

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NOTCH3(Trunc1)-Fc(DAPA)-AviTag-6xHis

Plasmid

#157045

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NOTCH3 (NOTCH3 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-VHC

Plasmid

#112618

Purpose

expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Venus (H2BC3 Aequorea victoria, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Tags

Venus

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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lentiMutB3GALT5catdead-blast

Plasmid

#220869

Purpose

lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

Depositor

Jennifer Kohler

Article

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

beta-1,3-galactosyltransferase 5 (B3GALT5 Human)

Use

Lentiviral

Tags

myc, FLAG

Expression

Mammalian

Mutation

encodes D156A and D243A mutations; also, nucleoti…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-H2B-iRFP670-p2a-YFP-Rb-IRES-blasticidin

Plasmid

#223962

Purpose

Dual fluorescent reporter for histone H2B and for exogenous Rb

Depositor

Hee Won Yang

Article

Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

Insert

H2B-iRFP670-p2a-YFP-Rb (RB1, H2B Human)

Tags

YFP and iRFP670

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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