We narrowed to 19,814 results for: INO

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pLKO.1 Sox2 3H b

Plasmid

#26352

Depositor

Matthew Meyerson

Article

Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

Insert

Sox2 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-PARP1_D770A-mCherry

Plasmid

#192260

Purpose

Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.

Depositor

Michael Cohen

Article

Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

Insert

PARP1_D770A (PARP1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D770A

Promoter

CMV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PARP1_R878A-mCherry

Plasmid

#192261

Purpose

Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.

Depositor

Michael Cohen

Article

Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

Insert

PARP1_R878A (PARP1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R878A

Promoter

CMV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

Plasmid

#105127

Purpose

Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein

Depositor

Kimberley Evason, Didier Stainier

Article

Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d

Inserts

Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)

Use

Zebrafish transgenesis

Promoter

cryaa (crystallin) and fabp10a

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

Plasmid

#65429

Purpose

Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

ChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Mutation

H134R variant

Promoter

hSyn1

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-ACE2-his expression plasmid

Plasmid

#158089

Purpose

Expresses ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

ACE2 (ACE2 Human)

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP12-5ptpase enzyme

Plasmid

#67515

Purpose

Recruitable human type IV 5-phosphatase enzyme

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Expression

Mammalian

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Promoter

CMV

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pMSCV-FLAG-2xSTREP-CDKN1B-Puro

Plasmid

#172611

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDKN1B (CDKN1B Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIK3R5

Plasmid

#23819

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3R5 (PIK3R5 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
CMV-SNAP-hCB2_IRES_EGFP

Plasmid

#223506

Purpose

Expression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

Depositor

James Frank

Article

Kosar et al J Am Chem Soc. 2023 Jul 19;145(28):15094

Insert

SNAP-tag-CNR2 and EGFP (CNR2 Human, Synthetic)

Tags

SNAP-tag

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-SpyTag-Flag-SRSF1-V5-mCherry

Plasmid

#235089

Purpose

SRSF1 mCherry fusion protein without MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-SpyTag-Flag-SRSF1-V5-mCherry (SRSF1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-h-cGASCD(K427E/K428E)

Plasmid

#127162

Purpose

expression of truncated human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

Plasmid

#209196

Purpose

Mutagenesis of Cnr1

Depositor

Larry Zweifel

Article

Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

Insert

Cnr1 (Cnr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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137_pAAV-ProA3-CatCh-GFP-WPRE

Plasmid

#125887

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA3

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-H874Y

Plasmid

#89087

Purpose

mammalian expression of human androgen receptor with prostate cancer somatic mutation H874Y from CWR22 xenograft

Depositor

Elizabeth Wilson

Article

Tan et al Mol Endocrinol. 1997 Apr;11(4):450-9. do

Insert

hAR-H874Y prostate cancer mutant (AR Human)

Expression

Mammalian

Mutation

hAR-H874Y

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pDONR223-PI4K2B

Plasmid

#23507

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PI4K2B (PI4K2B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
px330-Axin1

Plasmid

#162543

Purpose

sgRNA targeting Axin1

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Axin1 (Axin1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIK3R1

Plasmid

#23696

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3R1 (PIK3R1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PIK3C2G

Plasmid

#23483

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3C2G (PIK3C2G Human)

Use

Gateway donor vector

Mutation

C437T,3BP-del,1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PIK4CA

Plasmid

#23541

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK4CA (PI4KA Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only