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PLKO.1-UPF1-CDS

Plasmid

#136037

Purpose

UPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pIreSneo-FLAG/HA Ago3

Plasmid

#10823

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 3 (AGO3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

1 silent mutation

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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HCA2-Tango

Plasmid

#66396

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

HCA2 (HCAR2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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TFORF2061

Plasmid

#141900

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pVenus-C1-Cdc20 (1534)

Plasmid

#26058

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (Cdc20 Human)

Tags

Venus

Expression

Mammalian

Available Since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pGM

Plasmid

#71250

Purpose

luciferase reporter with human GM-CSF promoter -627 to + 28

Depositor

Peter Cockerill

Article

Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

Insert

GM-CSF promoter -627 to +28 fragment (CSF2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GM-CSF

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-8

Plasmid

#208786

Purpose

Inducibly expresses human Caspase 8 fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase 8 (CASP8 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

CARD domain is removed

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cGASsh4

Plasmid

#127646

Purpose

Knock-down of human cGAS

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

cGAS shRNA (CGAS Human)

Use

Lentiviral

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAT-WT-Myc

Plasmid

#177717

Purpose

ER protein alpha-1-antitrypsin (WT), myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Tags

Myc

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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sgTP53_3

Plasmid

#78164

Purpose

sgTP53

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pMK445 (BSD-P2A-BromoTag)

Plasmid

#214375

Purpose

BromoTag for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF1027

Plasmid

#141784

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RARA (RARA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

Plasmid

#67985

Purpose

Cas9 activity reporter (control) with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherryABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mRuby3

Plasmid

#155221

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Tags

mRuby3

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF3337

Plasmid

#144813

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HDAC1 (HDAC1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET SUMO NL-FKBP-Cas9

Plasmid

#162730

Purpose

Bacterial expression, protein expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Tags

6xHis and SUMO

Expression

Bacterial

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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GST-Rab8a-QL

Plasmid

#86079

Purpose

A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GST

Expression

Bacterial

Mutation

Changed glutamine 67 to leucine

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMyrPalm_mEGFP

Plasmid

#21037

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pMyrPalm

Tags

mEPFP

Expression

Bacterial and Mammalian

Available Since

May 12, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau AP P301L

Plasmid

#46906

Purpose

expresses EGFP tagged Tau AP P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP P301L (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

P301L AND all 14 S/P or T/P amino acid residues (…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_WT

Plasmid

#81923

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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