163,606 results

1. pPB-EF1a-MegaGate-DD-Hygro

   Plasmid

   #175267

   Purpose

   MegaGate destination with C-terminal DD tag and hygromycin resistance

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Tags

   Destabilization Domain

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMVV104

   Plasmid

   #215310

   Purpose

   Expresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   PAraBAD

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PX459-APP-KO

   Plasmid

   #176487

   Purpose

   To knockout APP gene

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   gRNA sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIB170

   Plasmid

   #90193

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EIF2AK3 gRNA (BRDN0001145848)

   Plasmid

   #77166

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EIF2AK3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EIF2AK3 (EIF2AK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pICSL50004

   Plasmid

   #50316

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, mCherry variant of RFP (Discosoma sp.)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA

   Plasmid

   #205281

   Purpose

   CaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons.

   Depositor

   Matthias Prigge

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a Gx-NL

   Plasmid

   #169105

   Purpose

   Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc
   

   Use

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKSJ155

   Plasmid

   #27126

   Depositor

   Alan Finkelstein

   Article

   Kienker et al J Gen Physiol. 2000 Oct . 116(4):587-98.

   Insert

   Colicin Ia + Ia immunity protein
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pJK225

    Plasmid

    #71124

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221_SLC16A9

    Plasmid

    #131876

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC16A9 (SLC16A9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pFA6a-hphMX6-Purg1-3FLAG

    Plasmid

    #19356

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    urg1 promoter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

    Plasmid

    #70191

    Purpose

    Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    ClpX (clpX )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EIF2AK3 gRNA (BRDN0001146328)

    Plasmid

    #77167

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIVT/Smarcc1

    Plasmid

    #122145

    Purpose

    For making Smarcc1 RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Smarcc1 (Smarcc1 Mouse)
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV Myc rat SAPAP3

    Plasmid

    #40217

    Depositor

    Yutaka Hata, Yoshimi Takai

    Article

    Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

    Insert

    SAPAP3 (Dlgap3 Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1(+)-RasAR(R89L)-NES

    Plasmid

    #205568

    Purpose

    RasAR negative-control mutant plasmid. Targeted to cytosol.

    Depositor

    Jin Zhang

    Article

    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

    Insert

    RasAR(R89L)-NES (RAF1 Human, Synthetic)
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Arg 89 mutated to Leu in Raf1 RBD region.

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS Wnt-4HA

    Plasmid

    #17375

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-4HA (Wnt4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pBS Wnt-5aHA

    Plasmid

    #17376

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-5aHA (Wnt5a Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pFA6a-KanMX6-Purg1-3FLAG

    Plasmid

    #19354

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    urg1 promoter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge