Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 61,522 results for: promoter

Clear keywords
Showing: 801 - 820 of 61522 results

  1. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139996-plasmid-map-sequence-id-275405

    Enlarge

  3. GFP-hPIKfyve

    Plasmid
    #121148
    Purpose
    Expresses GFP-tagged human PIKfyve in mammalian cells.
    Depositor
    Geert van den Bogaart
    Publication
    Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    121148-plasmid-map-sequence-id-237778

    Enlarge

  5. T7 promoter + terminators reporter plasmid

    Plasmid
    #156456
    Purpose
    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.
    Depositor
    Matthew D. Shoulders
    Publication
    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560
    Inserts
    NeoR/KanR
    TcR
    Expression
    Bacterial
    Mutation
    ATG start codon mutated to ACG
    Promoter
    Em7 and tet
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156456-plasmid-map-sequence-id-311167

    Enlarge

  8. pGP-AAV-syn-jGCaMP7f-WPRE

    Plasmid
    #104488
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has service
    AAV1, AAV8, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    jGCaMP7f
    Use
    AAV
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104488-plasmid-map-sequence-id-241289

    Enlarge

  9. pX330a dCas9-LSD1

    Plasmid
    #92362
    Purpose
    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-LSD1
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92362-plasmid-map-sequence-id-178233

    Enlarge

  10. pAAV.CAG.GCaMP6s.WPRE.SV40

    Plasmid
    #100844
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from CAG promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6s
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100844-plasmid-map-sequence-id-461658

    Enlarge

Showing: 801 - 820 of 61522 results