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pBABE-hygro-2xMyc-Rac1-P29S

Plasmid

#128581

Purpose

For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Changed Proline29 to Serine.

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1-V16-Luc

Plasmid

#122034

Purpose

AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

Insert

Luciferase

Use

AAV

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-eGFP-PC1-3HA

Plasmid

#108406

Purpose

Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Human)

Tags

3HA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_DOK1_WT

Plasmid

#82884

Purpose

Gateway Donor vector containing DOK1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DOK1 (DOK1 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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AiP12754 - pAAV-AiE0680m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2754)

Plasmid

#224004

Purpose

AiE0680m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L2/3_IT neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJAK184

Plasmid

#167281

Purpose

MazF-based mutagenesis vector for C. difficile

Depositor

Robert Fagan

Article

Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

Type

Empty backbone

Use

E. coli - c. difficile shuttle vector

Promoter

Pxyl

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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AiP11253 - pAAV-eHGT_017h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1253)

Plasmid

#163497

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJW31

Plasmid

#136423

Purpose

Contains genes of Type I-C CRISPR-Cas system for inducible expression and chromosomal integration at attTn7

Depositor

Joseph Bondy-Denomy

Article

Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

Inserts

Cas5
Cas8
Cas7
Cas3
Cas3

Use

CRISPR

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-GRET

Plasmid

#158222

Purpose

Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

GFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.E222

Plasmid

#138248

Purpose

Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
ECF20.N.(VDA).M86.N2
Terminator
SspGyrB.C2.(SAK).ECF16.C
Terminator
rhaS
ECF17.N.(NPC).NrdJ-1.N2
Terminator
M86.C2.(SDL).ECF20.C
Terminator
luxR
Terminator
ECF16.N.(AGG).SspGyrB.N2
Terminator
NrdJ-1.C2.(SEI).ECF17.C

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSET-dCas9-actII-4-NT-S1

Plasmid

#110185

Purpose

Repression of gene expression in Streptomyces by CRISPRi

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Inserts

dCas9
sgRNA

Use

CRISPR; I

Expression

Bacterial

Mutation

D10A, H840A

Promoter

J23119 and ermE*p

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB440

Plasmid

#74826

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR

Plasmid

#79162

Purpose

Expresses WspR WT under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB516

Plasmid

#74858

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB550

Plasmid

#74880

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Vx3(A7)

Plasmid

#35526

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3(A7)

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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pMAL-c2x-MT2

Plasmid

#32102

Depositor

David DeRosier, Chris Mercogliano, Cristina Risco Ortiz

Article

Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

Insert

MT (2x) (Mt1 Mouse)

Tags

MBP

Expression

Bacterial

Promoter

P-lac

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
LHP1480 - TOPO-TAP-tag

Plasmid

#32229

Depositor

Linda Hicke

Insert

TAP tag

Use

Cloning

Promoter

P-lac

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
His6tev CBP BCHZ (1079-1751)

Plasmid

#99341

Purpose

Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)

Tags

His6tev

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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HH02-LP Clone 2 heavy chain

Plasmid

#192175

Purpose

Clone 2 heavy chain (HH02)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 heavy chain (HH02)

Expression

Mammalian

Mutation

NA

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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