We narrowed to 9,126 results for: control

1. pmiRGLO-miR-124-sponge_rev(neg. CTRL)

   Plasmid

   #117326

   Purpose

   Dual luciferase assay negative control for miR-124 binding

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   reverse miR-124 sponge
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBacDual with LFn + FlaAAA

   Plasmid

   #84867

   Purpose

   Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   FlaAAA
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Insect

   Mutation

   3 C-terminal leucines have been replaced by alani…

   Promoter

   Polyhedrin

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. HcRed-pcw107-V5

   Plasmid

   #64647

   Purpose

   (control) when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   n/a
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   PGK

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pGL3-Tet1 promoter B

   Plasmid

   #63882

   Purpose

   Luciferase reporter plasmid used to study transcriptional control of murine Tet1

   Depositor

   Kian Peng Koh

   Article

   Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

   Insert

   Tet1 promoter fragment B
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a_MYC Ser62Ala_P2A_Hygro_Barcode

   Plasmid

   #120514

   Purpose

   Barcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC Ser62Ala (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Point mutation changing Serine to Alanine at amin…

   Promoter

   EF1a

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

   Plasmid

   #157691

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. EF1a_KLF11_P2A_Hygro_Barcode

   Plasmid

   #120497

   Purpose

   Barcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF11 (KLF11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKK-NoTag

   Plasmid

   #105767

   Purpose

   Control vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-RasAR(R89L)-NES

   Plasmid

   #205568

   Purpose

   RasAR negative-control mutant plasmid. Targeted to cytosol.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   RasAR(R89L)-NES (RAF1 Synthetic, Human)
   

   Use

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Arg 89 mutated to Leu in Raf1 RBD region.

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT-TO-FH-Nsp1

    Plasmid

    #157680

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pGL3-SV40P-Tet2 enhancer H1

    Plasmid

    #63883

    Purpose

    Luciferase reporter plasmid used to study transcriptional control of murine Tet2

    Depositor

    Kian Peng Koh

    Article

    Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

    Insert

    Tet2 enhancer fragment H1
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. JA740

    Plasmid

    #49939

    Purpose

    Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in EGFP for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD GFP (TET1 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pGL-p21UTRm1&2

    Plasmid

    #20880

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 1 (Position ~420) GC…

    Promoter

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

14. LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

    Plasmid

    #170254

    Purpose

    Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    A662C, N664C, V667L

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

    Plasmid

    #170222

    Purpose

    Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R24C

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TO-Nsp1-HF

    Plasmid

    #157681

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    HF-Nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH

    Plasmid

    #203841

    Purpose

    non-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    AAV

    Tags

    2A-mScarlet-KASH

    Expression

    Mutation

    G144A, Y145A, G146A

    Promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PA-RL-BAD

    Plasmid

    #78859

    Purpose

    DULIP positive control (bait) for assay establishment

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    BCL2 associated agonist of cell death (BAD Human)
    

    Use

    Expression vector

    Tags

    Protein A, Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a_HOXB6_P2A_Hygro_Barcode

    Plasmid

    #120454

    Purpose

    Barcoded lentiviral vector to express HOXB6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-FRT-TO-Nsp7-HF

    Plasmid

    #157690

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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