165,537 results

1. pFYF1548 EMX1

   Plasmid

   #47508

   Purpose

   human gRNA expression vector targeting EMX1

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EMX1 (EMX1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-GBF1

   Plasmid

   #206415

   Purpose

   Mammalian expression of hGBF1

   Depositor

   Kazuhisa Nakayama

   Article

   Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

   Insert

   GBF1 (GBF1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_Pro-IL-1α

   Plasmid

   #214341

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL1A (IL1A Human)
   

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKD-SepRS-EFSep

   Plasmid

   #34623

   Depositor

   Jesse Rinehart, Dieter Söll

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Inserts

   SepRS (MMP0688 Methanocaldococcuc maripaludis)
   EF-Sep (tufB E. coli)
   

   Expression

   Bacterial

   Mutation

   His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

   Promoter

   Ptac and Ptac (from SepRS- operon like)

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiGuide-Puro-P2A-EGFP_mRFPstuf

   Plasmid

   #137730

   Purpose

   Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6_rd6_pegRNA_(PP7-C4-Q1)

   Plasmid

   #232433

   Purpose

   pegRNA with optimized 3' modifications to correct the rd6 mutation

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T-1 3xHA-MEK1-DD

   Plasmid

   #47576

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MEK1-DD (Map2k1 Mouse)
   

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Ser218 to Asp, Ser221 to Asp

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pBIG2ab

   Plasmid

   #80616

   Purpose

   pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMB-SaCas9

   Plasmid

   #105998

   Purpose

   SaCas9 expression vector

   Depositor

   Mike McManus

   Article

   Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

   Insert

   Cas9 nuclease
   

   Use

   CRISPR and Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   short EF1alpha

   Available Since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gi)

    Plasmid

    #230019

    Purpose

    AAV vector expressing anti-mCherry PAGER(Gi) in neurons

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM1D
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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