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pXR004: CasRx pre-gRNA cloning backbone

Plasmid

#109054

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

Depositor

Patrick Hsu

Publication

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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OKSIM

Plasmid

#24603

Depositor

Jose Cibelli

Publication

Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

Insert

Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

Oct4-2A-KLF4-2A-Sox2-IRES-Myc

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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ABE8e

Plasmid

#138489

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ecTadA(8e)-nSpCas9

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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pSLCAR-CD19-BBz

Plasmid

#135992

Purpose

Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Publication

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pLV hUbC-Cas9-T2A-GFP

Plasmid

#53190

Purpose

3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

hUbC

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pINDUCER21 (ORF-EG)

Plasmid

#46948

Purpose

Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

Depositor

Stephen Elledge, Thomas Westbrook

Publication

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Type

Empty backbone

Use

Lentiviral

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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VCP(wt)-EGFP

Plasmid

#23971

Depositor

Nico Dantuma

Publication

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

none

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pEGFP hsp70

Plasmid

#15215

Depositor

Lois Greene

Publication

Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hsp70 (HSPA1A Human)

Tags

EGFP

Expression

Mammalian

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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pEGFP-VSVG

Plasmid

#11912

Depositor

Jennifer Lippincott-Schwartz

Publication

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VSVG

Expression

Mammalian

Mutation

F64L, S65T, A206K relative the wild type GFP sequ…

Available since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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