8,806 results

1. pRK5-p18-FLAG

   Plasmid

   #42331

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   p18 (LAMTOR1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xAP1pGL3 (3xAP-1 in pGL3-basic)

   Plasmid

   #40342

   Depositor

   Alexander Dent

   Article

   Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

   Insert

   3xAP-1
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Contains three canonical AP-1 binding sites (TGAC…

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-EF1-mVenus-p27K−

   Plasmid

   #176651

   Purpose

   It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).

   Depositor

   Mohamed Bentires-Alj

   Article

   Correia et al Nature. 2021 Jun;594(7864):566-571. doi:

   Insert

   mVenus-p27K- (Cdkn1b Mouse)
   

   Use

   Lentiviral

   Tags

   mVenus

   Mutation

   Mutant K- (lacks affinity to Cdk)

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1-FLAG-hSF3B1-K700E

   Plasmid

   #82577

   Purpose

   Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

   Depositor

   Manoj Pillai

   Article

   Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

   Insert

   human SF3B1-K700E (SF3B1 Human)
   

   Tags

   FLAG peptide

   Expression

   Mammalian

   Mutation

   K700E

   Promoter

   CMV

   Available Since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Flag mTOR wt

   Plasmid

   #26603

   Purpose

   Mammalian expression of human flag-tagged mTor

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   mTOR (MTOR Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. GSK3betaS9A  (1016)

   Plasmid

   #49492

   Purpose

   expresses human Gsk3beta with S9A (constitutively active) mutation

   Depositor

   Scott Friedman

   Article

   Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

   Insert

   GSK3beta S9A (GSK3B Human)
   

   Expression

   Mammalian

   Mutation

   S9A (constitutively active)

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET GFP LIC cloning vector (2GFP-T)

   Plasmid

   #29716

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, SiriusGFP, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCLBW cox8 EGFP mCherry

   Plasmid

   #78520

   Purpose

   Fluorescent mitophagy reporter

   Depositor

   David Chan

   Article

   Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

   Insert

   none
   

   Use

   Retroviral

   Tags

   cox8 EGFP mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. TOP-GFP

    Plasmid

    #35489

    Depositor

    Ramesh Shivdasani

    Article

    Horst et al Cancer Res. 2012 Feb 8.

    Insert

    7xTOP-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    7xTCF/LEF

    Available Since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSicoR human Dicer1

    Plasmid

    #14763

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Dicer shRNA (DICER1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cre Reporter

    Plasmid

    #62732

    Purpose

    Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    LoxP-DsRED-STOP-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    LoxP sites

    Expression

    Mammalian

    Promoter

    EF1-Alpha

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-BMPR2

    Plasmid

    #23669

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    BMPR2 (BMPR2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pRK5-HA-Ubiquitin-KO

    Plasmid

    #17603

    Purpose

    Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    KO: all lysines mutated to arginines

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. scFv-sfGFP-DNMT3A1

    Plasmid

    #102278

    Purpose

    The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

    Depositor

    Margaret Goodell

    Article

    Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

    Insert

    DNMT3A1 (DNMT3A Human)
    

    Use

    Lentiviral

    Tags

    scFV-sfGFP

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4-TO-Puromycin-mVenus-MAP

    Plasmid

    #44118

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Tags

    mVenus-MAP

    Expression

    Mammalian

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSicoR human Drosha1

    Plasmid

    #14766

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Drosha shRNA (DROSHA Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

18. Tag5Amyc-GSK3b KD

    Plasmid

    #16262

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    GSK3 beta (GSK3B Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Kinase dead: K85A

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMMP-MCS-IRES-eGFP

    Plasmid

    #36953

    Depositor

    Bill Sugden

    Article

    Kennedy et al Mol Cell Biol. 2003 Oct;23(19):6901-8.

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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