Skip to main content
Addgene
Showing: 2051 - 2100 of 18908 results
  1. pAAV-Ef1a-Con/Fon-Synaptophysin-10xMyc-WPRE

    Plasmid
    #237446
    Purpose
    Intersectional expression of Myc-tagged synaptophysin in the presence of Cre and Flp
    Depositor
    Insert
    Con/Fon-Synaptophysin-10xMyc
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. AAV-CAG-FLEX-LifeAct-jGCaMP8s-WPRE

    Plasmid
    #186049
    Purpose
    AAV-mediated expression of LifeAct-jGCaMP8s under the CAG promoter, Cre-dependent expression
    Depositor
    Insert
    LifeAct-jGCaMP8s
    Use
    AAV
    Tags
    LifeAct-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177211
    Purpose
    Expresses a Neomycin-targeting gRNA (mU6), a non-targeting gRNA (hU6) and Cre-recombinase
    Depositor
    Insert
    sgNeo/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. RV-Cag-Dio-mVenus

    Plasmid
    #153202
    Purpose
    Can be used to generate retrovirus that will mark the membrane (Palmitoylated-Venus) in the presence of Cre from the CAG promoter
    Depositor
    Insert
    membrane-tagged-Venus
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV-nEF Coff/Fon DREADD Gq-mCherry

    Plasmid
    #183534
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Flp but not Cre
    Depositor
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    None
    Promoter
    nEF
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV-nEF Con/Fon DREADD Gq-mCherry

    Plasmid
    #183532
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp
    Depositor
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    None
    Promoter
    nEF
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. hSyn-DIO-somBiPOLES-eGFP

    Plasmid
    #192584
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pAAV-nEF Con/Foff DREADD Gq-mCherry

    Plasmid
    #183533
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre but not Flp
    Depositor
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    None
    Promoter
    nEF
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. peAAV-CKII(0.4)-Chronos_eGFP_GP2A_nls_mScarlet

    Plasmid
    #121542
    Purpose
    Chronos with red nucleus-targeted fluorescence for excitatory neurons
    Depositor
    Insert
    blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    CamKII
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pDD288

    Plasmid
    #137017
    Purpose
    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Insert
    mNG-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mNG
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pAAV-synP-FLEX-TVA66T-EGFP

    Plasmid
    #64096
    Purpose
    Expresses TVA66T-EGFP under the synapsin promotor
    Depositor
    Insert
    TVA66T-EGFP
    Use
    AAV and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glutamic acid 66 to Threonine
    Promoter
    synapsin
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st

    Plasmid
    #177232
    Purpose
    Expresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgSik1_1st/sgSik2/sgSik3_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. bU6-sgMark2-mU6-sgMark3-hU6-sgMark4

    Plasmid
    #177235
    Purpose
    Expresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgMark2/sgMark3/sgMark4
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2

    Plasmid
    #177231
    Purpose
    Expresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgNT1/sgNeo2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pAAV-CAG-FLEX-TVA66T-EGFP

    Plasmid
    #64091
    Purpose
    Expresses TVA66T-EGFP under the CAG promotor
    Depositor
    Insert
    TVA66T-EGFP
    Use
    AAV and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glutamic acid 66 to Threonine
    Promoter
    CAG
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  16. pXP420 v2 (repaired Amp promoter)

    Plasmid
    #86920
    Purpose
    Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.
    Depositor
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  17. pDD291

    Plasmid
    #137019
    Purpose
    mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Insert
    mKate2-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mKate2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  18. bU6-sgNeo1-mU6-sgNT-hU6-sgSnrk

    Plasmid
    #177236
    Purpose
    Expresses neomycin (bU6), non-targeting (mU6) and Snrk (hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgNT1/sgSnrk
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  19. bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2

    Plasmid
    #177234
    Purpose
    Expresses Neomycin (bU6), Nuak1 (mU6) and Nuak2(hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgNuak1/sgNuak2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  20. bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2

    Plasmid
    #177233
    Purpose
    Expresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgPrkaa1/sgPrkaa2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  21. bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_2nd

    Plasmid
    #177230
    Purpose
    Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgNT1/sgLkb1_2nd
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  22. bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_1st

    Plasmid
    #177227
    Purpose
    Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgNT1/sgLkb1_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  23. pDD316

    Plasmid
    #137020
    Purpose
    mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms
    Insert
    mTurq2-GLO^SEC^2xHA + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    2xHA and C. elegans GLO-optimized mTurq2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  24. pBCSK_HIS3_II

    Plasmid
    #109059
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Insert
    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    HIS3
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
  25. pBCSK_HIS3_I

    Plasmid
    #109058
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Insert
    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    HIS3
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
  26. bU6-sgNeo1-mU6-sgLkb1_2nd-hU6-sgNT

    Plasmid
    #177229
    Purpose
    Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgLkb1_2nd/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  27. bU6-sgLkb1_1st-mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177225
    Purpose
    Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgLkb1_1st/sgNeo1/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  28. bU6-sgNeo1-mU6-sgLkb1_1st-hU6-sgNT

    Plasmid
    #177226
    Purpose
    Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgLkb1_1st/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  29. bU6-sgLkb1_2nd-mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177228
    Purpose
    Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgLkb1_2nd/sgNeo1/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  30. pDD289

    Plasmid
    #137018
    Purpose
    GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Insert
    GFP-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized GFP
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  31. pBCSK_LEU2_II

    Plasmid
    #109061
    Purpose
    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites downstream of LEU2. For teaching purposes.
    Depositor
    Insert
    LEU2 (LEU2 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    LEU2
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
  32. pAAV-TRE-DIO-hM4Di-mCherry

    Plasmid
    #182532
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.
    Depositor
    Insert
    AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    hM4Di-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  33. pAAV-TRE-DIO-Casp3-TEV

    Plasmid
    #183766
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.
    Depositor
    Insert
    AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  34. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid
    #220611
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.
    Depositor
    Insert
    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 2051 - 2100 of 18908 results