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Showing: 821 - 840 of 26688 results

  1. GFP-Cstem(7A)-Tac(5A)

    Plasmid
    #162499
    Purpose
    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    CD8a stem region with mutations and Tac full length with mutated stem region (IL2RA, CD8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five theronine and two serine residues are mu…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162499-plasmid-map-sequence-id-323491

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  2. AAV-KrasG13S/sgKras/Cre

    Plasmid
    #99859
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    99859-plasmid-map-sequence-id-200267

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  3. AAV-KrasG13A/sgKras/Cre

    Plasmid
    #99855
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    99855-plasmid-map-sequence-id-200262

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  4. MTST5

    Plasmid
    #86602
    Purpose
    Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.
    Depositor
    Mary Ann Moran
    Article
    Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1
    Insert
    MTST5
    Use
    Internal standard reverse transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    86602-plasmid-map-sequence-id-193516

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  5. MTST6

    Plasmid
    #86603
    Purpose
    Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.
    Depositor
    Mary Ann Moran
    Article
    Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1
    Insert
    MTST6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    86603-plasmid-map-sequence-id-193515

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  16. RSRF-Luc-2mut

    Plasmid
    #31819
    Depositor
    Astar Winoto
    Article
    Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.
    Insert
    Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    two CTATATTTAG RSRF sites mutated to CGATATTTCG
    Promoter
    mutated MEF2-dependent (-307 to -242 of Nur77/NR4…
    Available since
    Aug. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 821 - 840 of 26688 results