We narrowed to 3,098 results for: actin

1. Lifeact-mTFP1

   Plasmid

   #218980

   Purpose

   In vivo visualization of actin cytoskeleton

   Depositor

   Amro Hamdoun

   Insert

   Lifeact-mTFP1
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lifeact-mVenus

   Plasmid

   #218981

   Purpose

   In vivo visualization of actin cytoskeleton

   Depositor

   Amro Hamdoun

   Insert

   Lifeact-mVenus
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C3_SEPT6

   Plasmid

   #71583

   Purpose

   mammalian expression of human SEPT6 with a GFP fusion

   Depositor

   Elias Spiliotis

   Article

   Hu et al Curr Biol. 2012 Jun 19;22(12):1109-15. d

   Insert

   Septin 6 (SEPTIN6 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   V206D

   Promoter

   CMV

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-Anillin

   Plasmid

   #68027

   Purpose

   Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

   Depositor

   Michael Glotzer

   Article

   Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

   Insert

   Anillin (ANLN Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   silent mutations at 798 5'-TGCC TCTTTGAATAAA…

   Promoter

   CMV

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. HDAC8 Flag

   Plasmid

   #13825

   Depositor

   Eric Verdin

   Article

   Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

   Insert

   HDAC8 (HDAC8 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-Rab4a-7

   Plasmid

   #56440

   Purpose

   Localization: Ras GTPase, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

   Insert

   Rab4a (RAB4A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBa.M6PR-GFP

   Plasmid

   #224407

   Purpose

   Mammalian expression of M6PR

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   M6PR (M6PR Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-mouse vinculin full length (889)

   Plasmid

   #67935

   Purpose

   Mammalian expression of mouse vinculin with GFP fusion

   Depositor

   Alpha Yap

   Article

   Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

   Insert

   Vinculin (Vcl Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L159P in vinculin

   Promoter

   CMV

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBA-mKeima-PIM

   Plasmid

   #174650

   Purpose

   Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.

   Depositor

   Lukas Kapitein

   Article

   Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

   Insert

   xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

   Promoter

   Chicken beta-actin

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid

    #174644

    Purpose

    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    

    Use

    Tags

    Venus-SSPB and iLID-mCherry

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBa.Halo-Kif13B tail 442-1843

    Plasmid

    #162721

    Purpose

    Mammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residues

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Kif 13B (Kif13b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT with deletion of the 441 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJJR83

    Plasmid

    #75028

    Purpose

    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    mCherry^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized mCherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. plko-shmfn2-mito-GFP-ER tethering

    Plasmid

    #160509

    Purpose

    Rescue mfn2 knockdown by tethering mitochondria and ER with GFP

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    mito-GFP-ER tethering
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Myo1E-pEGFP

    Plasmid

    #124658

    Purpose

    Mouse myosin-1e fused with EGFP

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    Myo1e (Myo1e Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FERM domain of Ezrin

    Plasmid

    #62380

    Purpose

    Expresses the FERM domain of Ezrin (N terminal portion of the protein)

    Depositor

    Guillaume Charras

    Article

    Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

    Insert

    Ezrin aa1-309 (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains FERM domain of Ezrin (N terminal portion…

    Promoter

    CMV

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJJR82

    Plasmid

    #75027

    Purpose

    EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    EGFP^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized EGFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b_Q69L

    Plasmid

    #186272

    Purpose

    Expression vector of His-RanQ69L in E.coli

    Depositor

    Masahito Ikawa

    Article

    Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    GTP-binding nuclear protein Ran (Ran Mouse)
    

    Use

    Tags

    His-tagged at N terminal

    Expression

    Bacterial

    Mutation

    Changed Q69 into L

    Promoter

    T7 promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP TEM4 FL

    Plasmid

    #58893

    Purpose

    Expresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.

    Depositor

    Channing Der

    Article

    Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

    Insert

    TEM4 (ARHGEF17 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L722F

    Promoter

    CMV

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-N1-Cdc42BPB

    Plasmid

    #50759

    Purpose

    expression of EGFP tagged Cdc42BPB

    Depositor

    Naoki Mochizuki

    Article

    Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

    Insert

    Cdc42BPB (CDC42BPB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti CMVie-IRES-BlastR alt MCS

    Plasmid

    #120862

    Purpose

    3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

    Depositor

    Ghassan Mouneimne

    Article

    Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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