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pET22b-His6-UBCH7

Plasmid

#212711

Purpose

Bacterial expression of His6-UBCH7

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

UBCH7

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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A*24:02:01

Plasmid

#203295

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*24:02:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pP2-P1(S')

Plasmid

#196334

Purpose

Plasmid expressing chimeric P2-P1(S') tail fiber

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

chimeric tail fiber P2-P1(S') and chaperone protein U'

Expression

Bacterial

Promoter

P2 late promoter

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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PB_TRE_Z18-PDGFR_EF1a_eGFP

Plasmid

#186304

Purpose

Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

Z18-PDGFR
eGFP

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PD14

Plasmid

#164529

Purpose

Expresses HA-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin

Use

Lentiviral

Tags

HA and Ub

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SiC-V1-Scr

Plasmid

#133042

Purpose

SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

dTomato

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9D10A

Plasmid

#128161

Purpose

Ptet-controlled expression of S. pyogenes Cas9D10A variant

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Mutation

D10A

Promoter

Ptet

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-Cremyc-2A-GFP

Plasmid

#116879

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Article

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-26

Plasmid

#120005

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2-DRv6

Plasmid

#78628

Purpose

GESTALT V6 barcode for zebrafish

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V6

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pBLO1811_Cas9_noNLS_human

Plasmid

#74490

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO pTRE

Plasmid

#68457

Purpose

TOPO construct expressing pTRE for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Tetracycline Response Element Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAC-7-QFBDAD

Plasmid

#46096

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Expression

Insect

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe10_ChR2_mCherry

Plasmid

#213815

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_ccdB_lox_v2

Plasmid

#198040

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTCF120 H-2D(b)

Plasmid

#180454

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

H-2D(b)

Tags

BSP41

Expression

Bacterial

Mutation

Replacement of transmembrane domain with enzymati…

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP-HA_BASU

Plasmid

#153996

Purpose

Express eGFP gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

E-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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GP734

Plasmid

#164527

Purpose

Expresses Flag-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin

Use

Lentiviral

Tags

FLAG and Ub

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLCHKO

Plasmid

#155048

Purpose

Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTF101.2

Plasmid

#134771

Purpose

A binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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