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pHR-CMV-TetO2_3C-mVenus-Twin-Strep

Plasmid

#113891

Purpose

Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-mVenus-Twin-Strep

Promoter

CMV-MIE-TetO2

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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rLAMP1-mCherry-EGFP-Tet-On

Plasmid

#109015

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged mLAMP1 (lysosome marker)

Depositor

Jacob Corn

Publication

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

rLAMP1

Use

Lentiviral

Tags

mcherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSBY1_FnCpf1cg

Plasmid

#104622

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

Depositor

Sheng Yang

Publication

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

FnCpf1

Use

CRISPR

Expression

Bacterial

Mutation

codon-optimized C.glutamicum

Promoter

hsp60

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SMA-Cherry

Plasmid

#110209

Purpose

Mapping of mesenchymal progenitor cells.

Depositor

David Rowe

Publication

Grcevic et al Stem Cells. 2012 Feb;30(2):187-96. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Tags

None

Expression

Mammalian

Promoter

Smooth muscle alpha-actin and first intron

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBXNPHM3

Plasmid

#110099

Purpose

E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site

Depositor

Markus Seeger

Publication

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Tags

His

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pCDNA3-Flag-SPRTN-wt

Plasmid

#110214

Purpose

Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Publication

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Expression

Mammalian

Mutation

P296L (see depositor comments below)

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

Plasmid

#106204

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S + mRuby3 fusion

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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NA20 LN16

Plasmid

#101870

Purpose

Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

Depositor

Paul Clapham

Publication

Peters et al J Virol. 2004 Jul;78(13):6915-26.

Insert

Env (env )

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3-Venus-CAAX

Plasmid

#87612

Purpose

Plasma membrane-targeted Venus (YFP).

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Venus-CAAX

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SLC39A14-GFP

Plasmid

#104380

Purpose

Expresses SLC39A14/ZIP14 wild-type in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Publication

Liu et al J Biol Chem. 2017 Oct 6;292(40):16605-16

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SLC39A14 (SLC39A14 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCXN2-FLAG-AT2R-CFP

Plasmid

#101660

Purpose

Expresses AT2R with FLAG tag and CFP in mammalian cells.

Depositor

Yusuke Ohba

Publication

Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Angiotensin II type 2 receptor (AGTR2 Human)

Tags

ECFP and FLAG

Expression

Mammalian

Promoter

CAG promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHyg-AID*-9myc

Plasmid

#99518

Purpose

C-terminal AID*-9myc degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Publication

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDicAID_nCas9-PmCDA-2A-NptII_ETR

Plasmid

#91695

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223_LIG1_WT_V5

Plasmid

#83006

Purpose

Gateway Donor vector containing LIG1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

LIG1 (LIG1 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CFLAR_WT_V5

Plasmid

#82936

Purpose

Gateway Donor vector containing CFLAR, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CFLAR (CFLAR Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTlpA-Wasabi

Plasmid

#85455

Purpose

mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Publication

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA and TlpA, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 IRF7(5’UTR)-FF

Plasmid

#85490

Purpose

Firefly luciferase under the control of IRF7 5'UTR

Depositor

Ivan Topisirovic

Publication

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

5'UTR of IRF7 (Irf7 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAG416-Gal-PR50

Plasmid

#84901

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Publication

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_eGFP

Plasmid

#82714

Purpose

Expresses Myosin VI HMM with a C-terminal eGFP.

Depositor

Sivaraj Sivaramakrishnan

Publication

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

Myosin VI

Tags

FLAG purification tag and eGFP

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Publication

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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