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Plasmid#162984PurposeTet-inducible shRNA targeting human METTL3 #2DepositorInsertmethyltransferase like 3 (METTL3 Human)
UseLentiviralAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9-pAc
Plasmid#162163PurposeExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectableDepositorInsertshSpCas9
sgRNA
UseCRISPRTagsT2A-pAcExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEG BacMam C term GFP His8
Plasmid#160681PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTagsHis8 GFPExpressionMammalianPromoterCMVAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-pmExRai-AKAR2
Plasmid#161755PurposePlasma membrane-targeted ExRai-AKAR2.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn-ExRai-AKAR2
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1
Plasmid#119007PurposeMammalian expression of human BRCA1 and BFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRCA1 (BRCA1 Human)
ExpressionMammalianPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SPACE-NG (pRZ5150)
Plasmid#140244PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSPACE_NG
ExpressionMammalianMutationV82G in TadA*, NG mutations in SpCas9, D10A in Sp…PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF112
Plasmid#135920PurposeAll-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.DepositorTypeEmpty backboneUseLuciferase; TransposonExpressionMammalianAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5-TOM20_Halo_T2A_EGFP
Plasmid#135443PurposeMamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTOM20-HaloTag7 and EGFP
TagsTOM20ExpressionMammalianPromoterCMV/TetOAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ITPKA_3xmNeonGreen
Plasmid#129606PurposeIn vivo visualization of F-actin (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertF-tractin
ExpressionMammalianPromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX209-neo-WRN E84A K577M
Plasmid#125791Purposeconstitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRNDepositorInsertmyc-WRN E84A/K577M (WRN Human)
Tags5x mycExpressionMammalianMutationE84A/K577M (exonuclease/helicase-dead)Available sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-RAB-EKARev-NLS
Plasmid#118476PurposeddRFP-based single-color ERK biosensor targeted to the nucleus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAB-EKARev-NLS
Tags6xHIS, Nuclear localization signal (NLS), T7 tag …ExpressionMammalianPromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
MARIO/ pcDNA3
Plasmid#124838Purposemammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMARIO
TagsNo tagExpressionMammalianPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-3XNeon-nls
Plasmid#75386PurposePCP-3XNeon-nlsDepositorInsertPCP
UseLentiviralTags3XmNeonGreenExpressionMammalianPromoterEFSAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Myc-CyclinD1(WT)
Plasmid#122300Purposeexpresses Cyclin D1 protein with a Myc tagDepositorAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX-2T-GST-eIF4E K119A
Plasmid#112818PurposeExpresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cellsDepositorInsertEif4e (Eif4e Mouse)
TagsGSTExpressionBacterialMutationK119 mutated to A, increasing affinity for cap st…PromoterT7Available sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH-PABE-7-esgRNA
Plasmid#115620PurposeTargeted A to G in riceDepositorPublicationInsertwtTadA-TadA7.10-nCas9-3*NLS
UseCRISPRExpressionPlantAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSH231-EF1-BLST-dCas9-VPR
Plasmid#115148PurposeSafe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassetteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBlastR-P2A-dCas9-VPR
UseCRISPRTagsNucleoplasmin NLS and SV40NLSExpressionMammalianPromoterEF1aAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 3xFlag
Plasmid#110063PurposeFGFR2 expression in mammalian cells (JCOPCO paper)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfibroblast growth factor receptor 2 (FGFR2 Human)
TagsFlagExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 ermB cassette
Plasmid#117121PurposeErythromycin resistance cassetteDepositorInsertErythromycin resistance cassette for Bacillus subtilis selection
UseGolden gate donor vector for gene targeting in ba…Available sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only