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pOIMars1CyN

Plasmid

#75221

Purpose

Mammalian cell surface expression of fluorogen activating protein Mars1Cy

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

ss-HA-Mars1Cy-CD80(237-306)

Use

Tags

HA epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pEY105

Plasmid

#223452

Purpose

mgl-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

mgl-3 (mgl-3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEY86

Plasmid

#191079

Purpose

gcy-25 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-25 (gcy-25 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre_60.F2

Plasmid

#60208

Purpose

Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Human, Bacteriophage P1, Synthetic)

Use

Tags

FRAP and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 2021-2112 of FRAP with a T2098L mutat…

Promoter

CMV

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-DHFR

Plasmid

#80329

Purpose

Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

DHFR-TSc3

Use

CRISPR

Tags

Expression

Mutation

Promoter

DHFR-TS 5' UTR

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-Decoy

Plasmid

#80324

Purpose

Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

Decoy sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry 14-3-3β

Plasmid

#111880

Purpose

Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Use

Tags

Split mCherry system: 14-3-3β fused to C-terminal…

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry 14-3-3σ

Plasmid

#111881

Purpose

Mode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Use

Tags

Split mCherry system: 14-3-3σ fused to C-terminal…

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Ki-67(RASA)-mCherry_IRESpuro2

Plasmid

#182921

Purpose

Expresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.

Depositor

Daniel Gerlich

Article

Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

Insert

MKI67 (MKI67 Human)

Use

Tags

EGFP and mCherry

Expression

Bacterial and Mammalian

Mutation

RASA mutation

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCas9-CAT

Plasmid

#80323

Purpose

Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Inserts

Cas9
Chloramphenicol acetyltransferase

Use

CRISPR

Tags

3X FLAG and NLS

Expression

Mutation

Promoter

TgTUB1

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry NEDD4 WW2

Plasmid

#111882

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Use

Tags

Split mCherry system: NEDD4 WW2 fused to C-termin…

Expression

Bacterial

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenCMV-PMMars1CyAVPR2

Plasmid

#75226

Purpose

Lentiviral transfer vector encoding Mars1Cy-tagged AVPR2

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-AVPR2 (AVPR2 Synthetic)

Use

Lentiviral

Tags

HA epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-WT1shRNA

Plasmid

#220560

Purpose

To inducibly knockdown WT1 or EWSR1::WT1 expression

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

WT1 shRNA (WT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G (TetOP) promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEY80

Plasmid

#191075

Purpose

flp-18(AVA = 4.2-1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-18(AVA = 4.2-1k) (flp-18 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

Plasmid

#182918

Purpose

Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

Depositor

Daniel Gerlich

Article

Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

Insert

pH2B (H2BC16P Human)

Use

Tags

mNeonGreen-FRB(DmrC)

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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NET5s pEGFP-N2 (1154)

Plasmid

#62034

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET5s (TMEM201 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

short form

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pSALECT-Efnb2

Plasmid

#59707

Purpose

pSALECT with Efnb2 insert

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

Efnb2 (Efnb2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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MoFGFR31c-ires neo

Plasmid

#86496

Purpose

mamalian cell expression of FGFR3-FGFR1 chimera

Depositor

David Ornitz

Article

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR3-1 IIIc 15C7 (Fgfr1, Fgfr3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

FGFR3-FGFR1 chimera

Promoter

Moloney murine leukemia virus LTR

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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MoFGFR31b-ires neo

Plasmid

#86495

Purpose

mamalian cell expression of FGFR3-FGFR1 chimera

Depositor

David Ornitz

Article

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR31 IIIb 15C8 (Fgfr3, Fgfr1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

FGFR3-FGFR1 chimera

Promoter

Moloney murine leukemia virus LTR

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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