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hNav1.8_pcDNA3.1

Plasmid

#182847

Purpose

Expresses hNav1.8 in mammalian cells

Depositor

Paul DeCaen

Article

McCollum et al Cell Rep. 2022 Aug 23;40(8):111248. doi:

Insert

SCN10A (SCN10A Human)

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-C1

Plasmid

#37131

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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LV-GFP

Plasmid

#25999

Depositor

Elaine Fuchs

Article

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

H2B-GFP (H2BC21 Human, A. victoria)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

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3n1 (572)

Plasmid

#70727

Purpose

BetI/PhlF/SrpR BCD7 3-node v41-1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Inserts

srpR-ssrA
betI-ssrA
phlF-ssrA

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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AAVS1-PDL1

Plasmid

#159280

Purpose

Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

Human PDL1/CD274 (CD274 Human)

Use

AAV; S1 knockin donor vector

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-S33Y Beta-catenin

Plasmid

#19286

Purpose

Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S33Y (presumptive GSK3β phosphorylation site)

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire

Plasmid

#203658

Purpose

Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Tags

Sapphire

Expression

Yeast

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-PD-1-SNAP

Plasmid

#223576

Purpose

Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-mScarlet-WPRE

Plasmid

#130994

Purpose

AAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57.1_AOX-FLAG

Plasmid

#177984

Purpose

All-in-one dox-inducible expression of Alternative Oxidase

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

AOX

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Codon optimized cDNA for expression in human cells

Promoter

TRE

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-MCS-P2A-eGFP

Plasmid

#163356

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated GFP marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-CSNK1A1

Plasmid

#23355

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLKO_FOXA1_#1

Plasmid

#70095

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAS160

Plasmid

#194483

Purpose

synDHBR Cassette

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synDHBR Cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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modRNAc1-Cas9-2A-p53DD

Plasmid

#216389

Purpose

this vector can be used to make modRNA expressing Cas9 and p53DD

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

Cas9-2A-p53DD

Use

CRISPR

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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I48 ELP

Plasmid

#68937

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residue

Depositor

Andrew MacKay

Article

Shi et al J Control Release. 2013 Nov 10;171(3):33

Insert

I48 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-Hb9-RFP

Plasmid

#37081

Depositor

Fred Gage

Article

Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

Insert

Hb9 RFP

Use

Lentiviral

Promoter

Hb9

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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AAV pEF1a-DIO-FLPo-WPRE-hGHpA

Plasmid

#87306

Purpose

An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

Depositor

Li Zhang

Article

Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

Cre-dependent mouse codon-optimized Flp recombinase.

Use

AAV

Tags

N/A

Expression

Mammalian

Promoter

EF1a

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAdEasy-2

Plasmid

#16401

Purpose

Adenoviral vector for use in AdEasy System, for use with large inserts.

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP

Plasmid

#58472

Purpose

Expresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

Utr230 (UTRN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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