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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24,928 results for: crispr

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Showing: 8601 - 8620 of 24928 results

  8. pmiCas9

    Plasmid
    #162758
    Purpose
    express miCas9
    Depositor
    Eugene Chen
    Publication
    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miCas9
    Use
    CRISPR
    Tags
    FLAG and NLS
    Expression
    Mammalian
    Mutation
    Contains Brex27 sequence range is 4958-5065
    Promoter
    CMV
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    162758-plasmid-map-sequence-id-329432

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  9. pCgsgRNA_crtYf

    Plasmid
    #153338
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr
    Depositor
    Sheng Yang
    Publication
    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19
    Insert
    crRNA targeting crtYf
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153338-plasmid-map-sequence-id-306018

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  11. dABE

    Plasmid
    #124447
    Purpose
    Expresses dead Cas9 (dCas9)-ABE7.10
    Depositor
    George Church
    Publication
    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dABE7.10
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124447-plasmid-map-sequence-id-241059

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  12. dCBE4-gam

    Plasmid
    #124449
    Purpose
    Expresses dead Cas9 (dCas9)-CBE4-gam
    Depositor
    George Church
    Publication
    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCBE4-gam
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124449-plasmid-map-sequence-id-241051

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  13. pUBC_V5_MS2-mVenus

    Plasmid
    #107606
    Purpose
    Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag
    Depositor
    John Rinn
    Publication
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2-mVenus
    Use
    Synthetic Biology
    Tags
    V5 and mVenus
    Mutation
    MS2: V75E/A81G
    Promoter
    human UBC
    Available since
    May 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107606-plasmid-map-sequence-id-209713

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  18. pET-NlaCascade-NLS-6xHis

    Plasmid
    #180213
    Purpose
    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Insert
    NlaCas5-Cas8-Cas7
    Tags
    6XHis and NLS
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180213-plasmid-map-sequence-id-356902

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Showing: 8601 - 8620 of 24928 results