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pLAI-Env

Plasmid

#133996

Purpose

Env gene from HIV-1 LAI in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-BCL2

Plasmid

#116717

Purpose

Lentiviral expression of BCL2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BCL2 (BCL2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-AKAP-mtagBFP2-UBC6

Plasmid

#105007

Purpose

Mammalian Expression of mtagBFP2

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

mtagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-T2TP

Plasmid

#114725

Purpose

expression in mammalian cells

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

Tol2 transposase

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS578_DD-dSpyCas9-mCherry-APEX2

Plasmid

#108570

Purpose

Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Insert

dSpyCas9

Use

CRISPR and Lentiviral

Tags

APEX2, Destabilization domains, Flag, and mCherry…

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g1

Plasmid

#80784

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

Plasmid

#183409

Purpose

piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE3G

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 P-cad

Plasmid

#47502

Purpose

Expresses P-cadherin in mammalian cells

Depositor

Alice Wong

Article

Cheung et al Oncogene. 2010 Apr 22;29(16):2427-40. do

Insert

cadherin 3, type 1, P-cadherin (placental) (CDH3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-iGlu3slow1

Plasmid

#226501

Purpose

Superslow-decay iGluSnFR3 variant 1 for population imaging

Depositor

Katalin Torok

Article

Tran et al bioRxiv, August 30th, 2024

Insert

iGluSnFR3 D25A

Tags

IgK, Myc tag, and PDGFR Beta TM domain

Expression

Mammalian

Mutation

D25A

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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7TGC

Plasmid

#24304

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-eGFP // SV40-mCherry

Use

Lentiviral

Mutation

EGFP is GFP with S65T and F64L mutations

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pLL3.7-CAG-hMGT-Ef1a-puro

Plasmid

#172395

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pIVT-dCas9-mCherry

Plasmid

#174443

Purpose

in vitro transcription template for dCas9-mCherry

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Cas9 Endonuclease Dead

Use

In vitro transcription template

Tags

mCherry

Promoter

T7 SP6

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g4

Plasmid

#80787

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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mBaoJin-lifeactin

Plasmid

#229608

Purpose

Expresses mBaoJin-lifeactin in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hysn-flex-dsRed-shscramble

Plasmid

#71383

Purpose

Expresses scramble shRNA Cre-dependently by flex switch

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

human synapsin promoter-flex switch-dsRed-shscramble

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Promoter

human synapsin

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLM-CMV-R-Cre

Plasmid

#27546

Purpose

Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

Depositor

Michel Sadelain

Article

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

mCherry_P2A_Cre recombinase

Use

Cre/Lox and Lentiviral

Promoter

CMV

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pFLAG/HA-MOV10

Plasmid

#10976

Depositor

Thomas Tuschl

Article

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Insert

MOV10 (MOV10 Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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PGC-1 alpha promoter luciferase delta CRE

Plasmid

#8888

Depositor

Bruce Spiegelman

Article

Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

Insert

PGC-1 alpha promoter dCRE (Ppargc1a Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Site-directed mutagenesis to remove the CREB bind…

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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dCas12Max-ABE

Plasmid

#195335

Purpose

vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized dCas12Max-ABE

Expression

Mammalian

Mutation

N243R, E336R, D1049A

Promoter

CBh, CMV, hU6

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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CAG-BFP-sesRNA-GFP-WPRE-pA

Plasmid

#192063

Purpose

Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for tdTom

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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