159,557 results

1. MCS-TurboID MSCV-IRES-GFP

   Plasmid

   #207957

   Purpose

   Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminus

   Depositor

   Timothy Ley

   Article

   Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

   Insert

   TurboID
   

   Use

   Retroviral; Turboid biotin proximity ligase

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2474

   Plasmid

   #144029

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ONECUT3 (ONECUT3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_SMAD5_WT

   Plasmid

   #82217

   Purpose

   Gateway Donor vector containing SMAD5 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SMAD5 (SMAD5 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJB06

   Plasmid

   #190480

   Purpose

   Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

   Depositor

   Joseph Sorg

   Article

   Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

   Inserts

   Cas9
   XylR
   

   Use

   E. coli / b. subtilis - c. difficile shuttle vect…

   Tags

   Expression

   Mutation

   Promoter

   xylR-driven promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJB07

   Plasmid

   #190481

   Purpose

   Targeting plasmid for 2-plasmid C. difficile mutagenesis system

   Depositor

   Joseph Sorg

   Article

   Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

   Inserts

   Upstream & Downstream pyrE deletion region
   gRNA
   

   Use

   E. coli / b. subtilis - c. difficile shuttle vect…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

   Plasmid

   #210523

   Purpose

   AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   GFP-KASH
   U6-gRNA-directcapture
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRE-Tn7-145

   Plasmid

   #118561

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF1884

   Plasmid

   #143984

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZIC2 (ZIC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKnockinDonor-human-p16INK4A-P2A-mCherry

   Plasmid

   #137026

   Purpose

   donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

   Depositor

   Chunliang Li

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

   Insert

   p16INK4A-knockin-homology arm-P2A-mCherry
   

   Use

   Tags

   P2A-mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   no promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.4 human alpha5 full

    Plasmid

    #221396

    Purpose

    Mammalian expression of human integrin alpha5 full length

    Depositor

    Timothy Springer

    Article

    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

    Insert

    integrin alpha5 full length (ITGA5 Human)
    

    Use

    Tags

    CD33 secretion peptide (MPLLLLLPLLWAGALA) and St…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiV_Neo

    Plasmid

    #108101

    Purpose

    Lentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PRDX6A-c001

    Plasmid

    #175474

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    PRDX6 (PRDX6 Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    See Depositor Comments

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-DN-hCUL1-FLAG

    Plasmid

    #15818

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin1 (CUL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N452

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. FKBP-NTEVp(WT)

    Plasmid

    #137831

    Purpose

    Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-NTEVp(WT)
    

    Use

    Tags

    3x FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCM-CLYBL-hNIL

    Plasmid

    #105841

    Purpose

    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. FRB-CTEVp(WT)

    Plasmid

    #137832

    Purpose

    Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CTEVp(WT)
    

    Use

    Tags

    3x FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx4-H6-FGAm (FlincG3)

    Plasmid

    #49202

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    FlincG3
    

    Use

    Tags

    His tag + other tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Met to Lys in cpEGFP region similar to CGaMP3

    Promoter

    CMV

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-EGFP-Rac1(wt)

    Plasmid

    #13719

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    EGFP-Rac1(wt) (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR V5-RNF14_IRES-mCherry

    Plasmid

    #198384

    Purpose

    RNF14 expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RNF14 (RNF14 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGWB550

    Plasmid

    #74880

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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