We narrowed to 17,261 results for: tes
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Plasmid#229698PurposeTransient expression of GFP-alpha-cateninA- in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
TagsEGFPExpressionMammalianMutationL785A, I792A, V796APromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ptetO::bac::gfp
Plasmid#217882Purposeexpresses the bac-BGC in bacterial cells, produces bacillamide DDepositorInsertsDehydrogenase
Non-ribosomal peptide synthetase
Aminotransferase
ExpressionBacterialPromoterptetOAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)
Plasmid#216531PurposeTargeting vector for CRISPRd generationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABI-dCas9-mCherry
UseCRISPRTagsmCherryExpressionMammalianPromoterTRE3GAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA S27D
Plasmid#139194PurposeExpresses C-terminal HA tagged human Mfn2 S27D in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
Tags2HAExpressionMammalianMutationSerine 27 to Aspartic AcidPromoterCMV promoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRM053 His-TEV-Cas9 triNLS 0C
Plasmid#196244PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMDC32B-BS-AtMIR390a-B/c
Plasmid#199560PurposePlant expression vector (2x35S) for direct cloning of amiRNAs into Arabidopsis thaliana MIR390a precursor including only the basal stemDepositorInsertBasal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
UseRNAiPromoter2x35SAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJS288
Plasmid#200714PurposeTo express yeast Hxt1 hexose transporter in yeastDepositorUseSynthetic BiologyTagsmCherryExpressionYeastMutationdomesticated for MoClo cloning (removal of intern…Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag
Plasmid#185682PurposeLow-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERN1 (ERN1 Synthetic, Human)
TagsHaloTagExpressionMammalianMutationK599AAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRP1MX6-ins4
Plasmid#195041PurposepFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertTRP1
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…Available sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-MBD1 R44Q-14-mVenusC
Plasmid#191394PurposeR44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpcDNA3 Flag-MBD1 R44Q-14-mVenusC
Tags3xFLAGExpressionMammalianMutationR44QPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest-1.7kbfabp6-eGFP-pA-CrymCherry
Plasmid#159088PurposeLR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA insertsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseFluorescent reporterPromoter1.7kb fabp6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain
Plasmid#187275PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domainDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsmCherryMutationdeltaMyosin binding domainPromoterU6, CMVAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM9-BetaGal-8C
Plasmid#172489PurposePlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequenceDepositorInsertM9-BetaGal-8C
Tags6xHis and M9 nuclear localization sequence (from …ExpressionBacterialMutationBetaGal has two cysteine mutations (C77A, C1022S)…PromoterT7Available sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RP-CoV2/stSpike-puro
Plasmid#161794PurposeTransposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein productionDepositorInsertSARS-CoV-2 secretable trimeric Spike
UseTransposonTags6xHis and dTomatoExpressionMammalianPromoterEF1a/RPBSAAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast
Plasmid#166413Purposeexpress hPOT1 ΔOB in mammalian cellsDepositorInsertProtection Of Telomeres 1 (POT1 Human)
UseLentiviralTagsmycExpressionMammalianMutationΔOB deleted 125 a.aPromoteref1-aAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/flag-pBirA(1-G78)-FRB
Plasmid#154447Purposeexpresses pBirA(1-G78)-FRB/biochemical assayDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertflag-pBirA(1-G78)-FRB
ExpressionMammalianPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-SpCas9
Plasmid#130968Purpose3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xHA-NLS-SpCas9
UseCRISPRTags3x HA, EGFP, and NLSExpressionMammalianPromoterCbhAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)
Plasmid#129538Purposemodified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selectionDepositorTypeEmpty backboneUseCre/Lox; Mos1-mediated single copy insertion (mos…ExpressionWormAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-AD8-QES.i08.c04
Plasmid#123259PurposeMammalian expression plasmid for Env from the AD8 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopesDepositorInsertHIV-1 (AD8) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Mutations P124D, …PromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MA-VN
Plasmid#123283PurposeMammalian expression plasmid for matrix domain of HIV-1 Gag fused to the N-terminal half of split fluorescent Venus (VN)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMatrix
TagsVN: N-terminus of split Venus (a.a. V1–A154) I152…ExpressionMammalianPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only