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Showing: 8761 - 8780 of 13360 results

  1. INCTbiosyn-pEF-GFP(rc)phiC31

    Plasmid
    #127510
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    Expression
    Mammalian
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127510-plasmid-map-sequence-id-250757

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  8. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  9. pBFC0625

    Plasmid
    #186691
    Purpose
    VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_lacZ_α_1 Spacer crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186691-plasmid-map-sequence-id-371386

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  19. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid
    #122085
    Purpose
    Express M.EcoGII fused to CENPC with destabilization domain - inducible
    Depositor
    Laure Crabbe
    Article
    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5
    Insert
    DD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
    Use
    Retroviral
    Tags
    V5 tag on Nter of CENPC
    Expression
    Bacterial and Mammalian
    Mutation
    L341F, R930L (see comments below)
    Available Since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122085-plasmid-map-sequence-id-238127

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Showing: 8761 - 8780 of 13360 results