We narrowed to 7,454 results for: Lif

1. AAV-hSyn-jGCaMP7b-XC

   Plasmid

   #182221

   Purpose

   Improved genetic encoded calcium sensor based on GCaMP7b.

   Depositor

   Xiaodong Liu

   Article

   Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

   Insert

   jGCaMP7b-XC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAS12124_PEAR-GFP-preedited

   Plasmid

   #177179

   Purpose

   PEAR-GFP control plasmid with split EGFP and intact 5' splice site

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. OSCA3.1

   Plasmid

   #136596

   Purpose

   Expresses the mechanosensitive channel OSCA3.1 in mammalian cells

   Depositor

   Ardem Patapoutian

   Article

   Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

   Insert

   OSCA3.1 (ERD4 Mustard Weed)
   

   Use

   Tags

   pIRES2-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6b

   Plasmid

   #117222

   Purpose

   Expresses gRNA in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

   Insert

   HR U6b promoter-gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIC-lyn11-myc-LOV2GIVe

   Plasmid

   #170623

   Purpose

   For the mammalian expression of mLOV2GIVe

   Depositor

   Mikel Garcia-Marcos

   Article

   Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

   Insert

   Lyn11-myc-LOV2GIVe
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. MMLV-CAG-G_oT7pol-IRES-BSD

   Plasmid

   #172369

   Purpose

   Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   oT7pol + BSD
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MMLV-CAG-SADB19_oG-IRES-Puro

   Plasmid

   #172367

   Purpose

   Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   oG + Puro
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. U6c-T

   Plasmid

   #117211

   Purpose

   Expresses gRNA in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

   Insert

   u6c promoter-gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-51b(+)_NAD-Snifit

   Plasmid

   #113658

   Purpose

   Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NAD-Snifit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SAC1deltaTMD-FKBP-mCherry

    Plasmid

    #108123

    Purpose

    Recruitable cytosolic SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-520)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-Flag-wtSpCas9-H840A

    Plasmid

    #80453

    Purpose

    SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    SpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-SAC1-FKBP

    Plasmid

    #108129

    Purpose

    Recruitable SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:SACM1L:FKBP1A(3-108) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPBpuro-COX2-EBFP2

    Plasmid

    #184720

    Purpose

    Exogenous COX2

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    COX2-EBFP2 (PTGS2 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FKBP-AP180-GFP

    Plasmid

    #186577

    Purpose

    Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP180 (Snap91 Rat)
    

    Use

    Tags

    FKBP N-terminal GFP C-terminal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMA-SpCas9-g7

    Plasmid

    #80790

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. OSCA4.1

    Plasmid

    #136597

    Purpose

    Expresses the mechanosensitive channel OSCA4.1 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA4.1 (AT4G35870 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. tCRMod-GGSGGS9

    Plasmid

    #111762

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS9-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH-EF1a-mTagBFP-T2A-TetOn3G

    Plasmid

    #179888

    Purpose

    Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    TetOn3G
    

    Use

    Lentiviral

    Tags

    mTagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT-TetO-U2B''-fSNAPc

    Plasmid

    #106988

    Purpose

    open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    UB2" (SNRPB2 Human)
    

    Use

    Tags

    fSNAP

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET151-Vps4

    Plasmid

    #87733

    Purpose

    Bacterial expression of full-length S. cerevisiae Vps4

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. acta1-mRuby2-dGBP

    Plasmid

    #163855

    Purpose

    Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    dVHH[GFP]
    

    Use

    In vitro transcription of rna

    Tags

    Ruby2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Plas-gRNA-Z3EV

    Plasmid

    #157659

    Purpose

    GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    GFP-gRNA NatMX Z3EV
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-51b(+)_NADP-Snifit

    Plasmid

    #113913

    Purpose

    Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-TRE-DIO-proCaspase-3-TEVp

    Plasmid

    #166607

    Purpose

    Encodes Cre-dependent procaspase 3 and TEVp under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    procaspase3- TEVp
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCherry-FKBP-SAC1C389S

    Plasmid

    #108128

    Purpose

    Recruitable inactive SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(C389S)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-TRE-DIO-TVA-oG-mCherry

    Plasmid

    #166602

    Purpose

    Encodes Cre-dependent TVA-oG-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    TVA-oG-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. raACR-pmCerulean3-N1

    Plasmid

    #204959

    Purpose

    Expression of the MsACR1 mutant raACR as a fusion protein with mCerulean3 in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    raACR
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+enhancer)

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJFRC7-UAS-NPRR_ANP

    Plasmid

    #133243

    Purpose

    Fluorescent reporter for neuropeptide release imaging

    Depositor

    David Anderson

    Article

    Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

    Insert

    rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMA-SpCas9-g10

    Plasmid

    #80793

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge