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We narrowed to 5,876 results for: nls

Showing: 2151 - 2200 of 5876 results

  1. pY129 (pcDNA3.1-U6-huMb3Cpf1)

    Plasmid
    #92305
    Purpose
    Expression of humanized Mb3Cpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huMb3Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92305-plasmid-map-sequence-id-181650

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  2. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid
    #92303
    Purpose
    Expression of humanized TsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huTsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92303-plasmid-map-sequence-id-181636

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  3. pMG005_mNeonGreen_P2A

    Plasmid
    #237215
    Purpose
    Fluorescent reporter codon optimized for Dictyostelid species
    Depositor
    Jordi van Gestel
    Insert
    mNeonGreen-P2A
    Use
    Cloning
    Tags
    3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…
    Expression
    Mutation
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    237215-plasmid-map-sequence-id-473907

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  4. pMG008_mCherry_P2A

    Plasmid
    #237216
    Purpose
    Fluorescent reporter codon optimized for Dictyostelid species
    Depositor
    Jordi van Gestel
    Insert
    mCherry-P2A
    Use
    Cloning
    Tags
    3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…
    Expression
    Mutation
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    237216-plasmid-map-sequence-id-473906

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  7. pNB695

    Plasmid
    #60144
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Use
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Mutation
    Promoter
    MET17pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60144-plasmid-map-sequence-id-96226

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  8. pNB697

    Plasmid
    #60146
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    RdLuc
    Use
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Mutation
    Promoter
    MET17pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60146-plasmid-map-sequence-id-96253

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  9. pNB693

    Plasmid
    #60145
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    YeLuc
    Use
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Mutation
    Promoter
    MET17pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60145-plasmid-map-sequence-id-96228

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  10. pROF346

    Plasmid
    #155331
    Purpose
    Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.
    Depositor
    Matias Zurbriggen
    Article
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    RB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155331-plasmid-map-sequence-id-311105

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  11. pRD443

    Plasmid
    #168469
    Purpose
    For the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-mScarlet-I-H-NSdbd-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168469-plasmid-map-sequence-id-331930

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  12. pCMV-PE7-SB2

    Plasmid
    #228868
    Purpose
    PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence
    Depositor
    Sangsu Bae
    Insert
    PE7-2A-MLH1-SB-NLS-2A-MLH1-SB
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    228868-plasmid-map-sequence-id-456310

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  13. PX404 Campylobacter jejuni Cas9

    Plasmid
    #68338
    Purpose
    Campylobacter jejuni Cas9
    Depositor
    Feng Zhang
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    HA-NLS and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68338-plasmid-map-sequence-id-119561

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  14. pDSP33

    Plasmid
    #199375
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199375-plasmid-map-sequence-id-391013

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  15. pDSP32

    Plasmid
    #199374
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199374-plasmid-map-sequence-id-390801

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  16. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  17. pDSP31

    Plasmid
    #199373
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199373-plasmid-map-sequence-id-390590

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  18. pDSP23

    Plasmid
    #199372
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199372-plasmid-map-sequence-id-390928

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  25. lenti-dCas9-VP64-BFP

    Plasmid
    #196711
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-VP64-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196711-plasmid-map-sequence-id-392561

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  26. lenti-UCOE-dCas9-BFP-VP64

    Plasmid
    #196717
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196717-plasmid-map-sequence-id-388038

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  27. pLH_Scr16

    Plasmid
    #100538
    Purpose
    CEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Cdc1 NLS, CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    AA 1-621 of PhyB/ Mutation nucleotide 576 C-->A
    Promoter
    FBA1 and TDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100538-plasmid-map-sequence-id-194462

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  29. ePPE

    Plasmid
    #183095
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103
    Insert
    Nls-nCas9-NC-nls-MLV-Nls
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A in SpCas9
    Promoter
    Available since
    April 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183095-plasmid-map-sequence-id-358613

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  32. A3A-PBE

    Plasmid
    #119768
    Purpose
    Conversion of C to T in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    APOBEC3A-nCas9-NLS-UGI-NLS
    Use
    Tags
    NLS (flanking UGI)
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A and E945K in wild type …
    Promoter
    maize Ubiquitin-1
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119768-plasmid-map-sequence-id-232090

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  35. pH-A3A-PBE

    Plasmid
    #119774
    Purpose
    Conversion of C to T in rice plants transformation
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    APOBEC3A-nCas9-NLS-UGI-NLS
    Use
    Tags
    NLS (flanking UGI)
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A in wild type SpCas9
    Promoter
    maize Ubiquitin-1
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119774-plasmid-map-sequence-id-257661

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  36. BPK2139

    Plasmid
    #65776
    Purpose
    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65776-plasmid-map-sequence-id-112651

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  37. A3A-Gam

    Plasmid
    #119769
    Purpose
    Conversion of C to T monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS
    Use
    Tags
    NLS (flanking UGI)
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A in wild type SpCas9
    Promoter
    maize Ubiquitin-1
    Available since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    119769-plasmid-map-sequence-id-257611

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  40. lentiCRISPR.v2.TetON_Cas13d_U6_DR

    Plasmid
    #196727
    Purpose
    Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    TRE
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196727-plasmid-map-sequence-id-389689

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  41. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  42. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196712-plasmid-map-sequence-id-388051

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  43. pBUE411

    Plasmid
    #62200
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62200-plasmid-map-sequence-id-395472

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  45. pHSE401

    Plasmid
    #62201
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62201-plasmid-map-sequence-id-228092

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  46. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-131866

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  47. pHUE411

    Plasmid
    #62203
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62203-plasmid-map-sequence-id-467234

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  49. pBUN421

    Plasmid
    #62204
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    TaU3 and Ubi
    Available since
    Feb. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62204-plasmid-map-sequence-id-101817

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Showing: 2151 - 2200 of 5876 results