We narrowed to 14,443 results for: NTS

1. pcDNA3.1_mGFP-FGFR3-K650Q

   Plasmid

   #191756

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1948A>C (p.K650Q) + silent mutation c.1938C&…

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330-GAPDH

   Plasmid

   #136940

   Purpose

   NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

   Depositor

   Jeremy Stark

   Article

   Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

   Insert

   sgRNA targeting GAPDH (GAPDH Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX020

   Plasmid

   #167146

   Purpose

   MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   fungal caffeoylpiruvate hydrolase, nnCPH
   

   Use

   Synthetic Biology

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3

   Plasmid

   #106205

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Mutation

   GltI: A184V + mRuby3 fusion

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p21-WT-MEFV

   Plasmid

   #134702

   Purpose

   Lentiviral expression of human MEFV after doxycycline administration

   Depositor

   Thomas Henry

   Article

   Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

   Insert

   MEFV (MEFV Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Mutation

   WT

   Promoter

   TRE2 (Dox-inducible)

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Venus-Puma-pEGFP-C1

   Plasmid

   #166739

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Puma (BBC3 Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-SARS-CoV-2 Δ18 B.1.351v3

   Plasmid

   #169464

   Purpose

   Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

   Plasmid

   #149277

   Purpose

   Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Mutation

   T2318C (DNA) in PEBV coat protein sequence

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

   Plasmid

   #160624

   Purpose

   TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35S:MS2:VPR:Tnos
   

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-MTS-KD-c-Src-FLAG

    Plasmid

    #44653

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Tags

    FLAG tag and mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    changed lysine 298 to methionine

    Promoter

    CMV promoter

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.hSynap-FLEX.SF-iGluSnFR.A184V

    Plasmid

    #106181

    Purpose

    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-iGluSnFR.A184V
    

    Use

    AAV

    Mutation

    GltI: A184V

    Promoter

    hSynapsin

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9-plus

    Plasmid

    #126767

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLB052 - deAct - SpVB

    Plasmid

    #136286

    Purpose

    entry vector for GreenGate cloning method, D module, open reading frame of the mono(ADP-ribosyl) transferase domain of the Salmonella enterica SpvB

    Depositor

    Alexis Maizel

    Article

    Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

    Insert

    deAct - SpvB
    

    Expression

    Bacterial

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LV_EF1a_MAPK14-P2A-Hygro_Barcode

    Plasmid

    #170239

    Purpose

    Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    MAPK14 (MAPK14 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

    Plasmid

    #160627

    Purpose

    TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-MS2:TV-Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRES-hrGFP II-mTET1

    Plasmid

    #83569

    Purpose

    Expresses TET1 with a mutated catalytic domain in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Mutation

    catalytic domain mutant H1672D, D1674A

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A

    Plasmid

    #166804

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S1213A

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.hSynap-FLEX.SF-iGluSnFR.S72A

    Plasmid

    #106182

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Mutation

    GltI: S72A

    Promoter

    hSynapsin

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194377

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)
    

    Tags

    2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA6.2EmGFP-cDAXX

    Plasmid

    #169727

    Purpose

    Mammalian expression construct of GFP tagged cDAXX

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    cDAXX (DAXX Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Comprises residues 495-740

    Promoter

    CMV

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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