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pAGM55285

Plasmid

#153212

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shFEN

Plasmid

#17921

Depositor

Sheila Stewart

Article

Saharia et al Curr Biol. 2008 Apr 8. 18(7):496-500.

Insert

shFEN (FEN1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pTrichoGate-2

Plasmid

#160702

Purpose

gpdh promoter from Trichoderma virens bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

gpdh

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG mCherry-LDTS-eLIR

Plasmid

#189002

Purpose

lipophagy-inducing adaptor protein, AAV

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-eLIR, mCherry

Use

AAV

Tags

HA

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-LIC

Plasmid

#120247

Purpose

For LIC cloning with N-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSCL.139

Plasmid

#184994

Purpose

Express Cas9-P2A-Eco1RT in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

SpCas9-P2A-Eco1RT

Tags

SV40NLS

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-G9adSET

Plasmid

#41722

Purpose

Retroviral expression of human G9a mutant in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9adset (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt7A STOP

Plasmid

#35877

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7A (WNT7A Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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SAFB1-pcDNA3.1

Plasmid

#32742

Depositor

Steffi Oesterreich

Article

Townson et al J Biol Chem. 2003 May 30;278(22):20059-6

Insert

SAFB1 (SAFB Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pSLC-238

Plasmid

#73192

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-chpB (chpB )

Expression

Bacterial

Promoter

PrhaB

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pKPY514

Plasmid

#62598

Purpose

Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr251Gly-EcPheRS

Tags

6xHis

Expression

Bacterial

Mutation

Thr251Gly

Promoter

T5

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_NT135

Plasmid

#183121

Purpose

pLentiGuide modified to express mCherry and a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pNES-VL12.3

Plasmid

#43862

Depositor

Dane Wittrup

Article

Colby et al Proc Natl Acad Sci U S A. 2004 Dec 21;10

Insert

VL12.3

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt4

Plasmid

#35910

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Expression

Mammalian

Mutation

Silent Mutation C (492) --> T

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_Keratin

Plasmid

#82604

Purpose

Expresses sfCherry2(11) tagged keratin in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Keratin

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4D

Plasmid

#51599

Purpose

expresses mouse Sema4D with N-terminal myc tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D (Sema4d Mouse)

Tags

myc tag

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pDisplay FLIPE-600n

Plasmid

#13545

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Tags

ECFP and Venus

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pCBI-LexA-CIB1-biLINuS

Plasmid

#192605

Purpose

Plasmid for constitutive expression of LexA-CIB1-biLINuS.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCAGGS

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp6

Plasmid

#163590

Purpose

Expresses BMP-6 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp6 (Bmp6 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJL2-mGold-P2A-EBFP2

Plasmid

#157998

Purpose

Co-expression of mGold and EBFP2 in mammalian cells.

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold-P2A-EBFP2

Expression

Mammalian

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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