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L1-1180

Plasmid

#13265

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Expression

Bacterial

Mutation

Truncated after RSLE region

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
L1dRSLE

Plasmid

#13266

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Expression

Bacterial

Mutation

RSLE region removed

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
TK4_Zim3-dCas9-2xMyc-NLS-T2A-mApple_EF1a-Hygro

Plasmid

#254456

Purpose

TK4 PiggyBac plasmid for stable expression of Zim3-dCas9 during human pluripotent stem cell differentiation

Depositor

Marius Wernig

Article

Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2

Inserts

Zim3-dCas9-2xMyc-NLS-T2A-mApple
hygroR

Use

Piggybac

Tags

T2A-mApple

Expression

Mammalian

Promoter

CAG and EF1a

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-pTH-iCre-WPREpA

Plasmid

#213130

Purpose

Truncated rat TH promoter expressing iCre

Depositor

Ulrik Gether

Article

Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

Insert

iCre

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Promoter

Truncated TH promoter from rat (Rattus norvegicus…

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12556 - pAAV-AiE0410m_3xC-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2556)

Plasmid

#208144

Purpose

AiE0410m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain oligodendrocytes

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-tdTomato

Plasmid

#199250

Purpose

Fluorescent labelling of bacterial cells with tdTomato

Depositor

Uwe Mamat

Article

Mamat et al Appl Environ Microbiol. 2023 May 17:e003

Insert

Codon-optimized tdTomato gene

Expression

Bacterial

Mutation

Codon optimization for Xanthomonadaceae

Promoter

Pc promoter from class III integron of Delftia a…

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. aureus scaffold CARGO array

Plasmid

#191317

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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Cytosolic Fyn FRET Biosensor

Plasmid

#124654

Purpose

FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

Depositor

Yingxiao Wang

Article

Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

Insert

Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet

Expression

Mammalian

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-tTA

Plasmid

#162598

Purpose

human CD81-TEV cleavage site-tTA

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD81 (CD81 Human)

Tags

TEV protease cleavage site and tTA

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPN458

Plasmid

#137876

Purpose

PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

Plasmid

#127177

Purpose

N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

Flag- HA- Tandem Epitopes

Promoter

None

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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ECFP-IKB

Plasmid

#111193

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

IKB-alpha

Tags

ECFP

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pA

Plasmid

#37088

Purpose

Expresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic inhibition

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

NpHR3.0-EYFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hST3GAL6-RFP

Plasmid

#22923

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human type 2 lactosamine alpha-2,3-sialyltransferase promoter driving DsRedExpress (ST3GAL6 Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) rat C/EBP beta (LIP)

Plasmid

#12562

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta LIP (Cebpb Rat)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
Hmga2 3'UTR m2 luciferase (Luc-m2)

Plasmid

#14786

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Luc-m2 (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

m2 mutant (see article and author's map for …

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERP2-NR2F2

Plasmid

#192900

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of NR2F2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

NR2F2 (NR2F2 Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT (PGK) NanoLuc

Plasmid

#208576

Purpose

Empty NanoLuc Lentiviral reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

NanoLuciferase
d2eGFP
Empty response element

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

3x SV40 NLS and PEST

Expression

Mammalian

Promoter

PGK and miniP

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term SMN2

Plasmid

#187587

Purpose

Express FLAG epitope and APEX2-tagged SMN2 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SMN2 (SMN2 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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XLone-BSD SPI1

Plasmid

#179514

Purpose

Tunable and temporal expression control of SPI1

Depositor

Xiaoping Bao

Article

Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

Insert

SPI1 (SPI1 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GS Promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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