We narrowed to 9,370 results for: Pol;

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pPN105

Plasmid

#91634

Purpose

Express sgRNA targeting human KCTD13

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

KCTD13 (KCTD13 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2 - human TUBB2a Cterminal tail C127S, E435C

Plasmid

#60398

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking polyglutamate peptide

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C

Plasmid

#60405

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptide

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pEXP5-NT-7th R

Plasmid

#42573

Depositor

Ivan Korendovych

Article

Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

Insert

mutated calmodulin gene

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

changed Phenylalanine 92 to Glutamine acid, Isole…

Promoter

T7

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pFastBacHT (His10-4xFRB-SNAP-Lck G2A)

Plasmid

#177887

Purpose

Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Tags

10xHis-TEV-4xFRB-SNAP tag

Expression

Insect

Mutation

G2A

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

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pLJM1 flag-cFLIP

Plasmid

#211529

Purpose

Expresses flag-tagged cFLIP (CFLAR)

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

cFLIP (CFLAR Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2

Plasmid

#220243

Purpose

Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K63

Plasmid

#17606

Purpose

Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K63 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48

Plasmid

#17605

Purpose

Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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Ratiometric FPX sensor for ERK kinase activity

Plasmid

#60974

Purpose

For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A-WW domain-ERK substrate-ddFP B

Tags

ERK substrate is before ddFP copy B, WW domain is…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
pCMV4-ApoE4

Plasmid

#87087

Purpose

Expresses ApoE4 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

Apolipoprotein E4 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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p-EF1a-CreERT2-3Xflag-T2A-eBFP2

Plasmid

#170186

Purpose

This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

Depositor

Bradley Bernstein

Article

Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

Insert

CreERT2-T2A-eBFP2

Use

Lentiviral

Promoter

EF1 alpha

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV4-ApoE3

Plasmid

#87086

Purpose

Expresses ApoE3 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

Apolipoprotein E3 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMP71-hCD155

Plasmid

#118630

Purpose

human CD155 coding sequence subcloned into pMP71 retroviral plasmid

Depositor

Sébastien Walchli

Article

Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

Insert

CD155 (PVR Human)

Expression

Mammalian

Promoter

LTR

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV4-ApoE2

Plasmid

#87085

Purpose

Expresses ApoE2 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

Apolipoprotein E2 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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ZsGreen1-cMYC/pLVX-Puromycin

Plasmid

#180278

Purpose

Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

Depositor

Andre Catic

Article

Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

Insert

ZsGreen1-cMYC fusion protein MYC (MYC Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TAP DHX36

Plasmid

#53547

Purpose

mammalian expression of DHX36

Depositor

Kathleen Collins

Article

Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

Insert

DHX36 (DHX36 Human)

Tags

TAP

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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MOM603

Plasmid

#133242

Purpose

expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

Depositor

Shinsuke Niwa

Article

Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

Insert

kif1a (KIF1A Human)

Tags

StrepII tag and mScarlet

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

Plasmid

#208041

Purpose

Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

TurboID

Use

Lentiviral

Promoter

EFS

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTEF:ATP

Plasmid

#92179

Purpose

HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)

Use

Integration and expression into yeast.

Tags

Yeast codon optimized mseCFP (donor) and cp173-mV…

Expression

Yeast

Promoter

TEF1p

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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