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T_antiEGFPsynsrRNA_UA
Plasmid#185974PurposeExpresses antiEGFPsynsrRNA from PlTetO-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1.DepositorInsertPlTetO-1 AntiEGFPsynsrRNA
UseSynthetic BiologyTagsExpressionMutationPromoterPlTetO-1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
L_EGFP_AC
Plasmid#185971PurposeExpresses EGFP from PlLaco-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1DepositorInsertPl-LacO1-Egfp
UseSynthetic BiologyTagsExpressionMutationPromoterPlLacO-1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAT11
Plasmid#112592PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.DepositorTypeEmpty backboneUseTags6His-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD33
Plasmid#128286Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPara - lp-cfp-sopB
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKB253
Plasmid#170036PurposeEncodes LacI-controlled expression of surface-displayed K5L7 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
K5L7
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceMay 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PM mir shLEF1 b
Plasmid#27018DepositorInsertmir LEF1 1b
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-DHFRFLAG
Plasmid#63951PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAGExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-DHFRHIS
Plasmid#64003PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEE29
Plasmid#128287Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - sfyfp, sopC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-DHFRFLAG
Plasmid#64006PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAGExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMMDuet19
Plasmid#45594DepositorInsertNpuDnaB mini intein
UseTagsGB1 and H6-GB1ExpressionBacterialMutationremoval of endonuclease domainPromoterT7Available sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1mED
Plasmid#35033DepositorInsertFEN1mWT
UseAdenoviralTagsFlagExpressionMammalianMutationPT MUTANT E160DPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSPB867
Plasmid#128305Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPara - cfp-parB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-DHFRHIS
Plasmid#63985PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAT9
Plasmid#112590PurposeUsed for expression of protein in E. coli as Thrombin cleavable N-termina 6His-tag-MBP fusions and without NcoI site within MBP.DepositorTypeEmpty backboneUseTags6His-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PM mir shHoxB9 b
Plasmid#27020DepositorInsertmir HoxB9 3b
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-DHFRFLAG
Plasmid#63987PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAGExpressionBacterialMutationPromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-DHFRCYD
Plasmid#64005PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCYDExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-DHFRFLAG
Plasmid#63969PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAGExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHK-cinI
Plasmid#235486PurposeInducible gene cinI for OC14-HSL production by Ptac promoterDepositorArticleInsertcinI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK302.6
Plasmid#235471PurposeMessage phagemid carrying sgRNA6 (prom. J23110, backbone pBR322)DepositorArticleInsertsgRNA6
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK052.3
Plasmid#235472PurposeMessage phagemid carrying sgRNA3 (prom. J23119, backbone RSF1030)DepositorArticleInsertsgRNA3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK352.3
Plasmid#235473PurposeMessage phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)DepositorArticleInsertsgRNA3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.1
Plasmid#235460PurposeMessage phagemid carrying sgRNA1 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.2
Plasmid#235461PurposeMessage phagemid carrying sgRNA2 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.3
Plasmid#235462PurposeMessage phagemid carrying sgRNA3 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.4
Plasmid#235463PurposeMessage phagemid carrying sgRNA4 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.5
Plasmid#235464PurposeMessage phagemid carrying sgRNA5 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.6
Plasmid#235465PurposeMessage phagemid carrying sgRNA6 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA6
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only
