We narrowed to 7,454 results for: Lif

1. pMA-SpCas9-g8

   Plasmid

   #80791

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE-DIO-eGFP-L10a

   Plasmid

   #166605

   Purpose

   Encodes Cre-dependent eGFP-L10a under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   eGFP-L10a
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pF(UG) hSyn spCas9

   Plasmid

   #179725

   Purpose

   human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   spCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-DIO-GFP-2A-SynmRuby

   Plasmid

   #166608

   Purpose

   Encodes Cre-dependent GFP-2A-synaptophysinmRuby under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   GFP-2A-syn
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF(UG) hSyn SYT7alpha

   Plasmid

   #179713

   Purpose

   Lentiviral expression of mouse Syt7

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-mCherry-GS linker-Zdk1

   Plasmid

   #125226

   Purpose

   Expresses mCherry-GS linker-Zdk1 in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   mCherry-GS linker-Zdk1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-PB-PGK-tetR-KRAB-2A-Puro

   Plasmid

   #129536

   Purpose

   PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   PB-PGK-tetR-KRAB-2A-Puro
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   hPGK promoter

   Available since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT-TetO-U1-70K-fSNAPc

   Plasmid

   #106987

   Purpose

   open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   U1-70K (SNRNP70 Human)
   

   Use

   Tags

   fSNAP

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. tCRMod-GGSGGS7

   Plasmid

   #111761

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS7-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. tCRMod-GGSGGS5

    Plasmid

    #111760

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS5-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pgRNA-backbone

    Plasmid

    #172517

    Purpose

    Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

    Depositor

    Leonid Kruglyak

    Article

    Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

    Insert

    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMito-dCherry-FRB

    Plasmid

    #186573

    Purpose

    Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    Dark MitoTrap
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation of "K70N" to render mCherry no…

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. JB20

    Plasmid

    #128912

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab11AQL-BirA*-HA-MAO (RAB11A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-TK-Firefly-cjBCCIP

    Plasmid

    #122278

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjBCCIP 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOTTC1553 - pAAV EF1a EGFP-KASH

    Plasmid

    #131682

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Insert

    EGFP-KASH
    

    Use

    AAV

    Tags

    KASH

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV syn CCR6-eLOV-TEVcs-tTA

    Plasmid

    #173116

    Purpose

    Expresses CCR6 receiver construct in neurons

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    CCR6-eLOV-TEVcs-tTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRecLV103-GFP-PGBD5

    Plasmid

    #65409

    Purpose

    Lentiviral expression vector for fusion of GFP with human PGBD5

    Depositor

    Alex Kentsis

    Article

    Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

    Insert

    PGBD5 (PGBD5 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPRv2puro-Tyr

    Plasmid

    #170293

    Purpose

    A knock-out vector for the mouse tyrosinase.

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    A gRNA targeting the mouse tyrosinase gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Nephrin

    Plasmid

    #186134

    Purpose

    For expression of recombinant nephrin in bacteria cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    NPHS1 (NPHS1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Amino acid residues 1174-1223, with mutations: Y1…

    Promoter

    Lac

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUE1-TSP-ORF68

    Plasmid

    #162650

    Purpose

    Expresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pACS130

    Plasmid

    #204595

    Purpose

    Histone H3.1K9M-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    histone H3.1 (H3C3 Human)
    

    Use

    Lentiviral

    Tags

    human histone H3.1K9M-V5

    Expression

    Mammalian

    Mutation

    H3.1K9M

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJAG98-mTurquoise2-Nuf2

    Plasmid

    #80760

    Purpose

    Express Nuf2 N-terminally labeled with mTurquoise2

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    Nuf2 (NUF2 Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

    Plasmid

    #125231

    Purpose

    For generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirus

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. CALNL-RARa-VP16

    Plasmid

    #185560

    Purpose

    Cre Dependent ubiquitous expression of constitutively active Retinoic Acid Receptor alpha

    Depositor

    Connie Cepko

    Article

    Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

    Insert

    RARa-VP16 (Rara Mouse)
    

    Use

    Tags

    VP16

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

    Plasmid

    #87644

    Purpose

    For creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    myr::SNAPf>myr::CLIPf>myr::Halo2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. P303-FBP_2

    Plasmid

    #139693

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCS2-Opto-Alk8

    Plasmid

    #207615

    Purpose

    Zebrafish Alk8 BMP receptor kinase domain fused to VfLOV

    Depositor

    Patrick Müller

    Article

    Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

    Insert

    Alk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Zebrafish, Vaucheria frigida)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. JB93

    Plasmid

    #128904

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab7AQL-BirA*-HA-MAO (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. JB19

    Plasmid

    #128913

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab11ASN-BirA*-HA-MAO (RAB11A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1(+)-PM-CaNARi

    Plasmid

    #64850

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    Lyn-CaNARi
    

    Use

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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