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Showing: 2201 - 2250 of 2321 results

  2. pCRISPR-Cas9/CD4-TK2

    Plasmid
    #104397
    Purpose
    The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.
    Depositor
    Jeronimo Blanco
    Article
    Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39
    Insert
    TK2 (TK2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    104397-plasmid-map-sequence-id-208338

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  4. pcDNA3-eGFP-NLS-RNF168 delta RING

    Plasmid
    #133983
    Purpose
    mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)
    Depositor
    Daniel Durocher
    Article
    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.
    Insert
    RNF168 (RNF168 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    deletion of RING; siRNA resistant sequence: GAGGA…
    Promoter
    CMV
    Available since
    Nov. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    133983-plasmid-map-sequence-id-261913

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  6. pTT5-hSDC1-S15A

    Plasmid
    #52363
    Purpose
    expresses human Syndecan-1 serine 15 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S15A and R95Q (according to numbering in Fig. 2A …
    Promoter
    CMV
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pB-TR-TTHA

    Plasmid
    #232479
    Purpose
    Tetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Thermus thermophilus HB8
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TetO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232479-plasmid-map-sequence-id-471676

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  8. MKC84_HBB(HBA1reg-HBA1)

    Plasmid
    #232410
    Purpose
    AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.
    Depositor
    Kyle Cromer
    Article
    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232410-plasmid-map-sequence-id-463061

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  9. MKC83_HBB(HBBreg-HBA1)

    Plasmid
    #232409
    Purpose
    AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.
    Depositor
    Kyle Cromer
    Article
    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232409-plasmid-map-sequence-id-465033

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  10. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid
    #201677
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette
    Depositor
    Timo Otonkoski
    Article
    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-
    Insert
    MIR302-3g-EEA-2g-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201677-plasmid-map-sequence-id-395330

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  11. pGL3-pSMANTIS_1kb_KLF2mut 732

    Plasmid
    #194195
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 732)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194195-plasmid-map-sequence-id-381917

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  12. pGL3-pSMANTIS_1kb_KLF2mut 884

    Plasmid
    #194196
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 884)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 884)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194196-plasmid-map-sequence-id-381925

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  13. pGL3-pSMANTIS_1kb_KLF2mut 449

    Plasmid
    #194197
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 449)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 449)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194197-plasmid-map-sequence-id-381946

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  14. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)

    Plasmid
    #194191
    Purpose
    Includes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194191-plasmid-map-sequence-id-381776

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  15. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)

    Plasmid
    #194192
    Purpose
    Includes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    3 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194192-plasmid-map-sequence-id-381933

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  16. pGL3-pSMANTIS_1kb_KLF2mut 548

    Plasmid
    #194194
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 548)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 548)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194194-plasmid-map-sequence-id-381930

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  19. SIN40C.EFS.eGFP.mArid3a-3'UTR-WT

    Plasmid
    #169299
    Purpose
    Constitutive overexpression of the 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Depositor
    Jan-Henning Klusmann
    Article
    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0
    Insert
    Arid3a-3'UTR-WT (Arid3a Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169299-plasmid-map-sequence-id-345852

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  43. SIN40C.EFS.eGFP.ARID3A-3'UTR-WT

    Plasmid
    #169297
    Purpose
    Constitutive overexpression of the 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Depositor
    Jan-Henning Klusmann
    Article
    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0
    Insert
    ARID3A-3'UTR-WT (ARID3A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169297-plasmid-map-sequence-id-337857

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  45. pcDNA3-Flag::UbvG08 I44A, deltaGG

    Plasmid
    #74939
    Purpose
    Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    Ubiquitin
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    CMV
    Available since
    June 30, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    74939-plasmid-map-sequence-id-151347

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  47. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100087-plasmid-map-sequence-id-197556

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  48. pDmelOR

    Plasmid
    #126476
    Purpose
    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    DmelOrco (Orco Fly)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens; includes a rec…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126476-plasmid-map-sequence-id-246523

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  49. pMX-HA-UbvG08-IRES-GFP

    Plasmid
    #75030
    Purpose
    Retroviral vector with ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53)
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    Ubiquitin
    Use
    Retroviral
    Tags
    HA and IRES-eGFP
    Expression
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    Available since
    June 30, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    75030-plasmid-map-sequence-id-436413

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Showing: 2201 - 2250 of 2321 results